FastQCFastQC Report
Mon 16 Jan 2023
EGAF00007822542

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822542
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4606
Sequences flagged as poor quality0
Sequence length151
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT2645.731654363873209No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC1172.540165002171081No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT561.21580547112462No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT390.8467216673903605No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGCGTAATGTT290.6296135475466783No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGCGTAATGTTGTGGT240.5210594876248371No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGCGTAATGTTGTGGG210.4559270516717325No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC210.4559270516717325No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210.4559270516717325No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGCGTA190.4125054277029961No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGCGTAATGTTGTG180.39079461571862784No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG170.36908380373425964No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG140.303951367781155No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG140.303951367781155No Hit
CATCTGACGCTGCCGACGAGCGTAATGTTGTGGGGGTGTGGGGGGGGGGG130.2822405557967868No Hit
CGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130.2822405557967868No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAG120.26052974381241856No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC110.23881893182805036No Hit
CTTATACACATCTGACGCTGCCGACGAGCGTAATGTTGTGGGGGTGTGGG110.23881893182805036No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA110.23881893182805036No Hit
CATCTGACGCTGCCGACGAGCGTAATGTTGTGGGGGGGGGGGGGGGGGGG100.21710811984368217No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC100.21710811984368217No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT90.19539730785931392No Hit
CTTATACACATCTGACGCTGCCGACGAGCGTAATGTTGTGGGGGGGTGGG90.19539730785931392No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCA90.19539730785931392No Hit
CTTATACACATCTGACGCTGCCGACGAGCGTAATGTTGTGGGGGTGGGGG90.19539730785931392No Hit
GGATTCCCACCCGTCACACGCTACTCATGCCTGCATTCTCACTCGCATCA90.19539730785931392No Hit
GAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA90.19539730785931392No Hit
ATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90.19539730785931392No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGA90.19539730785931392No Hit
CATCTGACGCTGCCGACGAGCGTAATGTTGTGTAGATCTCGGTGGTCGCC80.17368649587494572Illumina RNA PCR Primer (95% over 22bp)
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG80.17368649587494572No Hit
CATCTGACGCTGCCGACGAGCGTAATGTTGTGGGGGTGGGGGGGGGGGGG80.17368649587494572No Hit
CTTATACACATCTGACGCTGCCGACGAGCGTAATGTTGTGGGGGGGGGGG80.17368649587494572No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80.17368649587494572No Hit
CTTATACACATCTGACGCTGCCGACGAGCGTAATGTTGTGGTGTTGTGGG70.1519756838905775No Hit
CATCTGACGCTGCCGACGAGCGTAATGTTGTGGTGGTGGGGGGGGGGGGG70.1519756838905775No Hit
ACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGCGTA70.1519756838905775No Hit
CTCTTATACACATCTGACGCTGCCGACGAGCGTAATGTTGTGGTGGTGTG70.1519756838905775No Hit
AGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA70.1519756838905775No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA70.1519756838905775No Hit
CTCTTATACACATCTGACGCTGCCGACGAGCGTAATGTTGTGGGGGTGTG70.1519756838905775No Hit
CTTATACACATCTGACGCTGCCGACGAGCGTAATGTTGTGGGGTTGTGGG60.13026487190620928No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGCGTAATGTTGTGTT60.13026487190620928No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATA60.13026487190620928No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGAA60.13026487190620928No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC60.13026487190620928No Hit
GCATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGCGTA60.13026487190620928No Hit
CATCTGACGCTGCCGACGAGCGTAATGTTGTGGTGTTGTGGGGGGGGGGG60.13026487190620928No Hit
CATCTGACGCTGCCGACGAGCGTAATGTTGTGGTGTGGTGGGGGGGGGGG60.13026487190620928No Hit
GGAACCCTTGGATAATCGGCGGACGGGATTCCCACCCGTCACACGCTACT60.13026487190620928No Hit
CCCATGGACTGTCTCCCGTAATTGAACTTTCAGTATTCGGAGTTTGCAAT60.13026487190620928No Hit
CATCTGACGCTGCCGACGAGCGTAATGTTGTGGGGTTGTGGGGGGGGGGG50.10855405992184108No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGCGTAATGTTGTGTA50.10855405992184108No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAA50.10855405992184108No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA50.10855405992184108No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGCA50.10855405992184108No Hit
CTTATACACATCTGACGCTGCCGACGAGCGTAATGTTGTGGTGTTGGGGG50.10855405992184108No Hit
CTCTTATACACATCTGACGCTGCCGACGAGCGTAATGTTGTGGGGGGGTG50.10855405992184108No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGA50.10855405992184108No Hit
CTCTTATACACATCTGACGCTGCCGACGAGCGTAATGTTGTGGTGTGGGG50.10855405992184108No Hit
TGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGCGTAATGT50.10855405992184108No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC50.10855405992184108No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGAAA50.10855405992184108No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC50.10855405992184108No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCA50.10855405992184108No Hit
GCAGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGAC50.10855405992184108No Hit
CCCCAGAAGCATCAAAAACACTCCCAAGACAAGCTCACATGCTTAGCATC50.10855405992184108No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)