FastQCFastQC Report
Tue 7 May 2024
EGAF00007822553

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822553
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11432
Total Bases1.7 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC59

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAGCTGTCAATCG5124.478656403079076No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC4654.067529741077677No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC3332.9128761371588525No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAGCTGTCAATCT3332.9128761371588525No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA840.7347795661301609No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG640.5598320503848845No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAGCTGTCAAT620.5423372988103569No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC590.5160951714485654No Hit
ATTGCGCAATGATCACGTTGGGGAAATCAACCGAGATTCCCTTAGTAGTG490.4286214135759272No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT480.4198740377886634No Hit
ATTGCGCAATGATGCATTTGGGGTGTCAGTGGTGAAGCTGCCGGGTGACT480.4198740377886634No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAGCTGTC450.39363191042687196No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT420.36738978306508047No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAG420.36738978306508047No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG410.35864240727781665No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT400.34989503149055284No Hit
ATTGCGCAATGCCGGCTACGGGGCACACCTAAATGGATTTATTGCCAATG380.33240027991602517No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACCCAGCTGTCAATCG330.2886634009797061No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC320.27991602519244224No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA310.27116864940517843No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG280.244926522043387No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT260.22743177046885937No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC260.22743177046885937No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT250.2186843946815955No Hit
ATTGCGCAATGACATTCGTGGGGGGTAACACTAACTGGAGGACCGAACCG250.2186843946815955No Hit
ATTGCGCAATGCAACACGGGGGGGAGAAGAGGAATAATAATAAGGGGCGG240.2099370188943317No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG220.19244226731980404No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAGC220.19244226731980404No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAGCTGTCAAGCG190.16620013995801258No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG190.16620013995801258No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC190.16620013995801258No Hit
ATTGCGCAATCTGTCTCTTATACACCTGGCTCTTATACACATCTCCGAGC180.15745276417074877No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG180.15745276417074877No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAG170.14870538838348496No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACCCAGCTGTCAATCT160.13995801259622112No Hit
ATTGCGCAATGCAACCATGGGGGGAGGTAGAGCTACTGGTTGGTTGAGCG160.13995801259622112No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAGCTGTCAATCTCG150.1312106368089573No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAGCTGTCAATGG150.1312106368089573No Hit
ATTGCGCAATGGCACATTTGGGGGGAGGGAGGTGAAGGAAAAAAAGAGAG140.1224632610216935No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTC140.1224632610216935No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTGT140.1224632610216935No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC130.11371588523442969No Hit
ATTGCGCAATGGCGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG130.11371588523442969No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC130.11371588523442969No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGT120.10496850944716585No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCA120.10496850944716585No Hit
ATTGCGCAATGTCAACTTGGGGGGGAGAAGAGGAATAATAATAAGGGGCG120.10496850944716585No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)