FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007822559

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822559
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10161
Sequences flagged as poor quality0
Sequence length151
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC4804.7239444936521995No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACCTATTGCATCG3583.523275268182265No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC2682.637535675622478No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACCTATTGCATCT2682.637535675622478No Hit
ATTGCGCAATGGGGGTATTGGGGGAAAAAGATATGATAGAATGAAAAAAA1611.584489715579175No Hit
ATTGCGCAATGGGCGTTAAGGGATTCTCGACGAAAGACGAGCTATGTGAG950.9349473477019978No Hit
ATTGCGCAATGGGGGTGAAGGGATTCTCGACGAAAGACGAGCTATGTGAG830.8168487353606929No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA780.7676409802184824No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACCTATTG580.5708099596496408No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG520.5117606534789882No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG510.5019191024505462No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC390.3838204901092412No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACCTATTGCAT380.37397893908079916No Hit
ATTGCGCAATGCTTCCAGTGGGCACTAAGCGAGCATCTGTTTCGGGATTT350.3444542859954729No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT350.3444542859954729No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT320.31492963291014664No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTACC280.2755634287963783No Hit
ATTGCGCAATGACAATCGAGGGGAAGCTTTTCGCATCCTGTCGTGCAACG270.2657218777679362No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACCT260.2558803267394941No Hit
ATTGCGCAATGAGGGTTCGGGGGGGTTACGGTGCCAACCTTCAACATTAC220.21651412262572584No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC220.21651412262572584No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTACCTATTGCATCG220.21651412262572584No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT210.20667257159728372No Hit
ATTGCGCAATGCAACGTTTGGGGTTATTGAGGGCGCATGGTGGATGCCTT200.19683102056884164No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA190.18698946954039958No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG180.1771479185119575No Hit
ATTGCGCAATGGGTCAGCAGGGGAAGTTGAAAATAAAAAAAAAAGAAAAA170.1673063674835154No Hit
ATTGCGCAATGGACCTCGCGGGCAGATTGGGAATATGTCCAGCATGAACT160.15746481645507332No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC160.15746481645507332No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC160.15746481645507332No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT150.14762326542663123No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTG150.14762326542663123No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG140.13778171439818915No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC130.12794016336974706No Hit
ATTGCGCAATGTATGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGA120.11809861234130499No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT120.11809861234130499No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGG120.11809861234130499No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC120.11809861234130499No Hit
GGGGGCTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG110.10825706131286292No Hit
ATTGCGCAATGAACTAGGCGGGCAGAAAGAAAATTAAAAAAAAGAAAAAG110.10825706131286292No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTC110.10825706131286292No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)