FastQCFastQC Report
Tue 7 May 2024
EGAF00007822563

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822563
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14033
Total Bases2.1 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC60

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCGCACAATCG6354.52504810090501No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC5964.247131760849427No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC4513.2138530606427707No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCGCACAATCT4423.149718520629944No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC1020.7268581201453717No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT870.6199672201239934No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCGCACAAT840.5985890401197178No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCGCAC830.5914629801182926No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG690.4916981400983397No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCT610.434689660086938No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT570.40618542008123715No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG530.37768118007553625No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACCTCTCGCACAATCG460.32779876006555975No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA420.2992945200598589No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT400.2850424000570085No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT400.2850424000570085No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTC380.27079028005415806No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC380.27079028005415806No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG320.22803392004560677No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC320.22803392004560677No Hit
ATTGCGCAATGGGGTCCAGGGGGAAGCAAAGTAGCAGCAGCACAACCAGC300.21378180004275635No Hit
ATTGCGCAATGCGATAGGAGGGTAAACATGAGAGTTTGATCTTGGCTCAG300.21378180004275635No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT290.20665574004133117No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG290.20665574004133117No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG290.20665574004133117No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC260.18527756003705553No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC250.17815150003563032No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT240.1710254400342051No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCGCA230.16389938003277987No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTGT220.15677332003135466No Hit
ATTGCGCAATGGTGATGTCGGGGGACGGACATGAACCCTAATAATTGTTT210.14964726002992945No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACCTCTCGCACAATCT210.14964726002992945No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTC200.14252120002850424No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCGCACAAGCG190.13539514002707903No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTG190.13539514002707903No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTGT190.13539514002707903No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGTC190.13539514002707903No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG190.13539514002707903No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA190.13539514002707903No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGCT180.12826908002565382No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCTC170.1211430200242286No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCGCACACTCG160.11401696002280338No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGT160.11401696002280338No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCGCACCATCG150.10689090002137817No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGT150.10689090002137817No Hit
ATTGCGCAATCTGTCTCTTATACACCTGGCTCTTATACACATCTCCGAGC150.10689090002137817No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)