FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007822564

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822564
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14033
Sequences flagged as poor quality0
Sequence length151
%GC58

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT7755.522696501104539No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC5423.8623245207724652No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACAGGAAGAGGGTGTA3562.5368773605073756No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACAGGAAGAGGGTGGT3032.1591961804318394No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACAGGAAGAGGGTGTT2341.6674980403334996No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACAGGAAGAGGGTGGA2151.5321029003064206No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACAGGAAGAGG810.5772108601154422No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGACAGGAAGAGGGTG800.570084800114017No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC710.50595026010119No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACAGGA580.41331148008266233No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACAGGAAGAGGGTGGG470.334924820066985No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACAGGAAGAGGGTGTG390.2779163400555833No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT390.2779163400555833No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC350.24941210004988243No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG330.235159980047032No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG320.22803392004560677No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA310.2209078600441816No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA290.20665574004133117No Hit
TGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACAGGAAGAG270.19240362003848074No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC250.17815150003563032No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA240.1710254400342051No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC230.16389938003277987No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCA230.16389938003277987No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG220.15677332003135466No Hit
GATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACAGGAA220.15677332003135466No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT210.14964726002992945No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG190.13539514002707903No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAA180.12826908002565382No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATA180.12826908002565382No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC180.12826908002565382No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGC160.11401696002280338No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGCA150.10689090002137817No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAAA150.10689090002137817No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)