FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007822577

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822577
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19021
Sequences flagged as poor quality0
Sequence length151
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACATCGCATATCG7213.790547289837548No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC6503.4172756427106883No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACATCGCATATCT5252.7601072498817096No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC3912.0556227327690446No Hit
ATTGCGCAATGTTCGTAGTGGGGCTCTTTCGGCCTTGCTTGCCGGCAGAC1640.8622049313916198No Hit
ATTGCGCAATGATGATGCGGGGGGGCGCATGGTGGATGCCTTGGCAACAG1580.8306608485358289No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG1210.6361390042584512No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACATCGCA1080.5677934914042374No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACATCGCATAT970.5099626728352873No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC940.49419063140739183No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT890.46790389569423263No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTACA880.4626465485516008No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA750.39430103569738706No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT720.37852899426949166No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC590.31018348141527785No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT570.2996687871300142No Hit
ATTGCGCAATGGGAGTCAAGGGGAAGGGGGGGTGCAGAAGGGTAGGCACC560.29441143998738234No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG540.2838967457021187No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTACATCGCATATCG520.27338205141685507No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA510.26812470427422325No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG490.25761000998895955No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT450.23658062141843225No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC420.2208085799905368No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC390.20503653856264129No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG380.19977919142000947No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACAT360.18926449713474583No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAC350.18400714999211398No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGT310.16297776142158668No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTACATCGCATATCT270.14194837285105935No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC260.13669102570842753No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTC260.13669102570842753No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT260.13669102570842753No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT250.13143367856579571No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC250.13143367856579571No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG240.12617633142316387No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTGT230.12091898428053204No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGATG230.12091898428053204No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCT200.10514694285263657No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)