FastQCFastQC Report
Tue 7 May 2024
EGAF00007822579

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822579
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14239
Total Bases2.1 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC5623.946906383875272No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATAGGTTGGATCG4663.2727017346723786No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC3602.5282674345108505No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATAGGTTGGATCT3482.4439918533604885No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG950.6671816841070299No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATAGGTTGGAT730.5126764519980336No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTATA710.4986305218063067No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA670.4705386614228527No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT600.4213779057518084No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG600.4213779057518084No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATAGGTTG590.4143549406559449No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC540.3792401151766276No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT470.33007935950558326No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT440.30901046421799283No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC370.2598497085469485No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA360.252826743451085No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG350.2458037783552216No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT320.22473488306763117No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATAG290.20366598778004072No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTATAGGTTGGATCG290.20366598778004072No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG280.19664302268417727No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT240.16855116230072337No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC230.1615281972048599No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACTATAGGTT230.1615281972048599No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGTC230.1615281972048599No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAT220.15450523210899642No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCTT210.14748226701313294No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG200.14045930191726946No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC200.14045930191726946No Hit
ATTGCGCAATGTCAGGCCGGGGGTGGATGCCTTGGCGATATCAGGCGATG190.13343633682140602No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTC180.1264133717255425No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT180.1264133717255425No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC180.1264133717255425No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATAGGTTGGATCG160.11236744153381559No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGCT160.11236744153381559No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATAGGGTGGATCT160.11236744153381559No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC150.1053444764379521No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTGT150.1053444764379521No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATAGGGTGGATCG150.1053444764379521No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGT150.1053444764379521No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)