FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007822580

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822580
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14239
Sequences flagged as poor quality0
Sequence length151
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCCGCATTAGTGTA9776.8614368986586145No Hit
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT7125.0003511482547935No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC4603.230563944097198No Hit
CTTATACACATCTGACGCTGCCGACGATTCCGCATTAGTGTAGATCTCGG1270.8919165671746612No Hit
CTCTTATACACATCTGACGCTGCCGACGATTCCGCATTAGTGTAGATCTC950.6671816841070299No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCCGCATTAGTG870.6109979633401221No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC800.5618372076690779No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCCG720.50565348690217No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCCGCATTA630.4424468010393989No Hit
CATCTGACGCTGCCGACGATTCCGCATTAGTGTAGATCTCGGTGGTCGCC500.3511482547931737Illumina Single End PCR Primer 1 (100% over 22bp)
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT470.33007935950558326No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT460.3230563944097198No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC430.30198749912212935No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA400.2809186038345389No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT380.26687267364281203No Hit
CTTATACACATCTGACGCTGCCGACGATTCCGCATTAGTGTAGTTCTCGG370.2598497085469485No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG340.2387808132593581No Hit
ATACACATCTGACGCTGCCGACGATTCCGCATTAGTGTAGATCTCGGTGG330.23175784816349462No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC320.22473488306763117No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA300.2106889528759042No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG280.19664302268417727No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA270.1896200575883138No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGATTCCGCATTAGTGTAGA260.18259709249245032No Hit
CTCTTATACACATCTGACGCTGCCGACGATTCCGCATTAGTGTAGTTCTC240.16855116230072337No Hit
TGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCCGCATT240.16855116230072337No Hit
GATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCCGC230.1615281972048599No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC230.1615281972048599No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC220.15450523210899642No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCC220.15450523210899642No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGC220.15450523210899642No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATAT210.14748226701313294No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC210.14748226701313294No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAA210.14748226701313294No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG200.14045930191726946No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG200.14045930191726946No Hit
CATCTGACGCTGCCGACGATTCCGCATTAGTGTAGATCTCGGTGGTGGCC180.1264133717255425Illumina Single End PCR Primer 1 (95% over 22bp)
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATA180.1264133717255425No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAT180.1264133717255425No Hit
CATCTGACGCTGCCGACGATTCCGCATTAGTGTAGATCTCGGTGGGCGCC160.11236744153381559Illumina Single End PCR Primer 1 (95% over 22bp)
ATACACATCTGACGCTGCCGACGATTCCGCATTAGTGTAGATCTCGGGGG160.11236744153381559No Hit
CTTATACACATCTGACGCTGCCGACGATTCCGCATTAGTGTAGATCGCGG160.11236744153381559No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGCAA160.11236744153381559No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCG160.11236744153381559No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATATGTG150.1053444764379521No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGCAAA150.1053444764379521No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150.1053444764379521No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)