FastQCFastQC Report
Mon 16 Jan 2023
EGAF00007822584

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822584
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3281
Sequences flagged as poor quality0
Sequence length151
%GC52

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCCGCATTAGTGTA2367.192928985065529No Hit
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT1263.840292593721426No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC631.920146296860713No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC190.5790917403230723No Hit
CTCTTATACACATCTGACGCTGCCGACGATTCCGCATTAGTGTAGATCTC180.5486132276744895No Hit
CTTATACACATCTGACGCTGCCGACGATTCCGCATTAGTGTAGATCTCGG160.48765620237732393No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCCGCATTA160.48765620237732393No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCCGCATTAGTG130.3962206644315757No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130.3962206644315757No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGC120.365742151782993No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG120.365742151782993No Hit
CATCTGACGCTGCCGACGATTCCGCATTAGTGTAGATCTCGGTGGTCGCC110.33526363913441026Illumina Single End PCR Primer 1 (100% over 22bp)
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG110.33526363913441026No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100.3047851264858275No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCCG100.3047851264858275No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT90.27430661383724475No Hit
ACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCCG90.27430661383724475No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCCGCATTAGTGGA80.24382810118866197No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCC80.24382810118866197No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGCAA70.21334958854007927No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAAA70.21334958854007927No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC70.21334958854007927No Hit
CATCTGACGCTGCCGACGATTCCGCATTAGTGTAGATCTCGGTGGGGGCC70.21334958854007927No Hit
CTTATACACATCTGACGCTGCCGACGATTCCGCATTAGTGTAGTTCTCGG70.21334958854007927No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATAT70.21334958854007927No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATA70.21334958854007927No Hit
GATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCCGC60.1828710758914965No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG60.1828710758914965No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG60.1828710758914965No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC60.1828710758914965No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA60.1828710758914965No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA60.1828710758914965No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAA50.15239256324291375No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATATG50.15239256324291375No Hit
CATCTGACGCTGCCGACGATTCCGCATTAGTGTAGATCTCGGTGGGGGGC50.15239256324291375No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGAG50.15239256324291375No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCG50.15239256324291375No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC50.15239256324291375No Hit
GCCCTATACGTCGTCTTGAAGCCGACTTAGCAGAGCCCTGTGTTTTTGCT50.15239256324291375No Hit
TGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCCGCATT40.12191405059433098No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAGC40.12191405059433098No Hit
GCCGTATATTGTTACATATTGTCACTACCTCCCCGTGTCGGGATTGGGTA40.12191405059433098No Hit
CATCTGACGCTGCCGACGATTCCGCATTAGTGTAGATCTCGGTGGTGGCC40.12191405059433098Illumina Single End PCR Primer 1 (95% over 22bp)
CATCTGACGCTGCCGACGATTCCGCATTAGTGTAGATCTCGGTGGGCGCC40.12191405059433098Illumina Single End PCR Primer 1 (95% over 22bp)
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATGTGT40.12191405059433098No Hit
CATCTGACGCTGCCGACGATTCCGCATTAGTGTAGATCTCGGTGGTCGGC40.12191405059433098Illumina Single End PCR Primer 1 (95% over 22bp)
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAT40.12191405059433098No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCA40.12191405059433098No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA40.12191405059433098No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGATTCCGCATTAGTGTAGA40.12191405059433098No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGCAAT40.12191405059433098No Hit
ATACACATCTGACGCTGCCGACGATTCCGCATTAGTGTAGTTCTCGGTGG40.12191405059433098No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAA40.12191405059433098No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCCCAAAA40.12191405059433098No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAAA40.12191405059433098No Hit
TTTTTTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA40.12191405059433098No Hit
GTCTCTTATACACATCTGACGCTGCCGACGATTCCGCATTAGTGTAGATC40.12191405059433098No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATA40.12191405059433098No Hit
CTTATACACATCTGACGCTGCCGACGATTCCGCATTAGTGTATTTCTCGG40.12191405059433098No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATC40.12191405059433098No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGCA40.12191405059433098No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCA40.12191405059433098No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGCAAAAT40.12191405059433098No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC40.12191405059433098No Hit
ATACACATCTGACGCTGCCGACGATTCCGCATTAGTGTAGATCTCGGTGG40.12191405059433098No Hit
GTGCTGATCTTCCTTTTCCCATCCGTGGCTCTCAACAGACACTTGTTTTC40.12191405059433098No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)