FastQCFastQC Report
Tue 7 May 2024
EGAF00007822587

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822587
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12773
Total Bases1.9 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC58

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCGCTCGTCATCG4423.460424332576529No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC3843.006341501604948No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCGCTCGTCATCT3322.5992327565959443No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC2642.0668597823534016No Hit
ATTGCGCAATGGTGGGTCCGGGGCTTTTGTGACTGGCGGTGAACGAGTGC940.7359273467470445No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG930.7280983324199484No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA830.6498081891489861No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC800.6263211461676975No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT740.5793470602051202No Hit
ATTGCGCAATGGGATGGCGGGGGGGAGTAATTTCGGAATATAGAGGTGTG670.5245439599154467No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCGCTCGT650.5088859312612543No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT550.4305957879902921No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT550.4305957879902921No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCG530.4149377593360996No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCGCTCGTCAT480.3757926877006185No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT450.35230564471932985No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA390.3053315587567525No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTTCGCTCGTCATCG360.2818445157754639No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC330.2583574727941752No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG300.23487042981288653No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC290.22704141548579035No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG280.21921240115869411No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG270.21138338683159788No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC250.19572535817740547No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCGC250.19572535817740547No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTGT240.18789634385030926No Hit
CCGGTACACAAAGCTGACCGACGCCCAGATCCTCACCAGGCTGATGAATG230.18006732952321303No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGCT230.18006732952321303No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC230.18006732952321303No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG230.18006732952321303No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG220.17223831519611682No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT220.17223831519611682No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC210.1644093008690206No Hit
ATTGCGCAATGCAATGAAGGGGGGCAAAATCAAAGCTTGACAAAGGGAGT200.15658028654192438No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCGCTCGTCATCGCG180.14092225788773194No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTG180.14092225788773194No Hit
ATTGCGCAATGCTAGGCAGGGGGGGACCATTCCATTCAGGAGCAAAGAGC180.14092225788773194No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTC170.1330932435606357No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT170.1330932435606357No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCT160.1252642292335395No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGTC150.11743521490644326No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGT150.11743521490644326No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGT140.10960620057934706No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCGCTCG140.10960620057934706No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTG140.10960620057934706No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGGC140.10960620057934706No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTC130.10177718625225085No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCG130.10177718625225085No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGC130.10177718625225085No Hit
ATTGCGCAATGTATGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGA130.10177718625225085No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTGT130.10177718625225085No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCGCTCGTCATCTCG130.10177718625225085No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)