FastQCFastQC Report
Tue 7 May 2024
EGAF00007822591

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822591
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13800
Total Bases2 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACATGGACGATCG5964.318840579710145No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACATGGACGATCT3912.833333333333333No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC3882.8115942028985508No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC2491.8043478260869563No Hit
ATTGCGCAATGTTTGTATCGGGGATATTGAACTCGAGTTGGAAGAGGCGA1901.3768115942028984No Hit
ATTGCGCAATGTTACGCCTGGGATCTTTGGGGTGTGGCTGAAGTCGAAAA1010.7318840579710144No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG870.6304347826086957No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACATGGAC780.5652173913043479No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA770.5579710144927535No Hit
ATTGCGCAATGTAATCCGCGGGAGGGATTGCCTCAGTAACGGCGAGTGAA740.5362318840579711No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC720.5217391304347827No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACATGGACGAT660.47826086956521735No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT620.4492753623188406No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT580.4202898550724638No Hit
ATTGCGCAATGTTGCACTCGGGGGGGTGGAAAGAAGAGTAAGGGTAGAGG530.38405797101449274No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTACA510.3695652173913043No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT410.2971014492753623No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA390.28260869565217395No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC390.28260869565217395No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT360.26086956521739135No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTACATGGACGATCG350.25362318840579706No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACAT320.2318840579710145No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG300.21739130434782608No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC290.2101449275362319No Hit
ATTGCGCAATGCGTCGGCGGGGGGGCTTCCGCCGGCGCCAGTGGCCTAGC280.2028985507246377No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG260.18840579710144928No Hit
ATTGCGCAATGCGGTGGTTGGGGATCGTAGTCCGGATTGCACTCTGCAAC260.18840579710144928No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC250.18115942028985507No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC250.18115942028985507No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACATGGACGAGCG240.17391304347826086No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG230.16666666666666669No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT200.14492753623188406No Hit
ATTGCGCAATGTTGACTTCGGGGGGGTGGAAAGAAGAGTAAGGGTAGAGG200.14492753623188406No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTGT200.14492753623188406No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG200.14492753623188406No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC180.13043478260869568No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT170.12318840579710144No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGTC170.12318840579710144No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACATGGACGAGCT170.12318840579710144No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTACATGGACGATCGCG170.12318840579710144No Hit
ATTGCGCAATGGCAGCAAGGGGGGGGTCAAGCGAACAAGTGCATGTGGTG160.11594202898550725No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTACATGGACGATCT160.11594202898550725No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG160.11594202898550725No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTC150.10869565217391304No Hit
ATTGCGCAATGTATGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGA150.10869565217391304No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGCT150.10869565217391304No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGT150.10869565217391304No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGT140.10144927536231885No Hit
GGGGGCTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG140.10144927536231885No Hit
ATTGCGCAATGTATGCTCTGTCTCTTATACACATCTCCGAGCCCACGAGA140.10144927536231885No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAC140.10144927536231885No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTCT140.10144927536231885No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGGC140.10144927536231885No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)