FastQCFastQC Report
Thu 19 Jan 2023
EGAF00007822597

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822597
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29756
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCACGGATCG7042.3659093964242506No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC4951.663530044360801No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCACGGATCT3971.3341847022449254No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC2780.9342653582470762No Hit
ATTGCGCAATGAGATGTGGGGGGTTCCTATAGGGGAGGTGGTGGCAGGTA2280.7662320204328539No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2130.7158220190885872No Hit
ATTGCGCAATGTATCCCTGGGGGACAACCTTAGCCAAACCATTTACCCAA1730.5813953488372093No Hit
ATTGCGCAATGTAGCTGGGGGGAGTAGAGGCTAAAGTGAGCTGACATTGC1630.5477886812743648No Hit
ATTGCGCAATGGTCAAACAGGGGCAAAATAGTGGGAAGATTTATAGGTAG1520.510821346955236No Hit
ATTGCGCAATGTATGCTTAGGGGTGGGAAGATTTATAGGTAGAGGCGACA1440.48393601290496036No Hit
ATTGCGCAATGGTGTTTCCGGGGAAACTGCGAATGGCTCATTAAATCAGT1420.4772146793923914No Hit
ATTGCGCAATGTGCACGCCGGGGCAAAATAGTGGGAAGATTTATAGGTAG1400.47049334587982256No Hit
ATTGCGCAATGGCTAGTAGGGGATGTAGCAAAATAGTGGGAAGATTTATA1380.4637720123672536No Hit
ATTGCGCAATGGCGGCGCTGGGGCAAAATAGTGGGAAGATTTATAGGTAG1370.46041134561096925No Hit
ATTGCGCAATGGCACTCTCGGGGTAACAAAATAGTGGGAAGATTTATAGG1350.4536900120984003No Hit
ATTGCGCAATGCGCCGACAGGGTTACTGAACTGGCGGAAGATAAAGAGAA1340.4503293453421158No Hit
ATTGCGCAATGCTACTCCGGGGTAGCTCTTTCTCGATTCCGTGGGTGGTG1340.4503293453421158No Hit
ATTGCGCAATGGGATGAAAGGGATAGTGGGAAGATTTATAGGTAGAGGCG1310.4402473450732625No Hit
ATTGCGCAATGCTCTCCTGGGGTAGAATCTTAGTTCAACTTTAAATTTGC1270.4268046780481248No Hit
ATTGCGCAATGCCACTCTGGGGATAATACGCCTCACACTCATTCTCAACC1260.4234440112918403No Hit
ATTGCGCAATGAGGTTAACGGGGTGGGAAGATTTATAGGTAGAGGCGACA1250.4200833445355558No Hit
ATTGCGCAATGCTCTCTTCGGGCTAAATCCCCTTGTAAATTTAACTGTTA1230.413362011022987No Hit
ATTGCGCAATGCTGGCCTGGGGGAATCTTAGTTCAACTTTAAATTTGCCC1210.40664067751041805No Hit
ATTGCGCAATGTATCCAGGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGC1190.3999193439978492No Hit
ATTGCGCAATGCCTGGGTCGGGAGTTCAACTTTAAATTTGCCCACAGAAC1180.39655867724156474No Hit
ATTGCGCAATGGGGTTCGTGGACAAACCTACCGAGCCTGGTGATAGCTGG1180.39655867724156474No Hit
ATTGCGCAATGCGAAGGAGGGGCAGTTGACACAAAATAGACTACGAAAGT1170.39319801048528025No Hit
ATTGCGCAATGTTAAGACTGGGGGGAAGATTTATAGGTAGAGGCGACAAA1140.38311601021642694No Hit
ATTGCGCAATGCGCCTCAAGGGGAGAGCGGTCTCCGGAAGGCTCCGCCAC1110.37303400994757363No Hit
ATTGCGCAATGTATACCCTGGGCTGATGTTCGCCGACCGTTGACTATTCT1100.36967334319128914No Hit
ATTGCGCAATGAAGGGCGGGGGATCAACCTGTACACTGTCATCTTCATTA1080.36295200967872027No Hit
ATTGCGCAATGGCTAGAGTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGC1070.3595913429224358No Hit
ATTGCGCAATGGTTTGAACGGGTAGCCAATATTGTGCCTATTGCCATACT1030.34614867589729803No Hit
ATTGCGCAATGCCACAAAGGGGTGGGTCGTTCATCCCCGGTGGTGGCTGC1000.3360666756284447No Hit
ATTGCGCAATGCGTGGGGTGGGCACGGGTGACGGGGAATCAGGGTTCGAT980.3293453421158758No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG950.31926334184702243No Hit
ATTGCGCAATGGGTTGGCCGGGGGCTTTGGTGACTCTAGATAACCTCGGG940.315902675090738No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCACG930.31254200833445356No Hit
ATTGCGCAATGACTTCACGGGGATGGTTCCTTTGGTCGCTCGCTCCTCTC930.31254200833445356No Hit
ATTGCGCAATGCTCTACTCGGGGGCGACAAACCTACCGAGCCTGGTGATA920.30918134157816907No Hit
ATTGCGCAATGCGCGCACCGGGGGATCCCTTCGCCAGGTACAGGGCGCAA900.3024600080656002No Hit
ATTGCGCAATGCCGAAGAGGGGGATACCCCACTATGCTTAGCCCTAAACC880.2957386745530313No Hit
ATTGCGCAATGGATAATGAGGGGGTTTGGTCCTAGCCTTTCTATTAGCTC880.2957386745530313No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC860.2890173410404624No Hit
ATTGCGCAATGTTCGGTCTGGGGCAGCAGGCGCGCAAATTACCCACTCCC830.2789353407716091No Hit
ATTGCGCAATGTCACACGGGGGTCGATGGTAGTCGCCGTGCCTACCATGG800.2688533405027557No Hit
ATTGCGCAATGTAGTATCGGGGCAAAACCAACCCGGTCAGCCCCTCTCCG790.26549267374647134No Hit
ATTGCGCAATGGTACCCCGGGGCAGTTGACACAAAATAGACTACGAAAGT780.26213200699018685No Hit
ATTGCGCAATGGCAATAGAGGGGTGGGAAGATTTATAGGTAGAGGCGACA750.25205000672133354No Hit
ATTGCGCAATGATTGGATGGGGAGATTAAGCCATGCATGTCTAAGTACGC740.24868933996504908No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCACGGAT730.24532867320876459No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCA720.24196800645248018No Hit
ATTGCGCAATGACATAGTGGGGAAGCATAATATAGCAAGGACTAACCCCT690.2318860061836268No Hit
ATTGCGCAATGCATAATACGGGCGAGCCTGGTGATAGCTGGTTGTCCAAG690.2318860061836268No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT680.22852533942734238No Hit
ATTGCGCAATGCTCATTTGGGGTGAATTCCACGGTCATCTAACAGAACTG640.2150826724022046No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT640.2150826724022046No Hit
ATTGCGCAATGTTGGGTGGGGGGCAGTCGGGTCCCGGCAGCGGCTGCAGC630.21172200564592014No Hit
ATTGCGCAATGTCGAGGCGGGGGTGGGAAGATTTATAGGTAGAGGCGACA610.20500067213335124No Hit
ATTGCGCAATGGTTCCTTGGGGGTTGGTTAAAAATCACATCAAAAAGCTA600.20164000537706683No Hit
ATTGCGCAATGAAGAATGGGGGCAACTTCTTAGAGGGACAAGTGGCGTTC600.20164000537706683No Hit
ATTGCGCAATGAACTCTGCGGGCGCCCTGCTATCTTCTGGCTTTTCCCTT580.1949186718644979No Hit
ATTGCGCAATGACAAACCGGGGGATCCCTTCGCCAGGTACAGGGCGCAAA570.19155800510821347No Hit
ATTGCGCAATGACGGGTATGGGATAGTGGGAAGATTTATAGGTAGAGGCG570.19155800510821347No Hit
ATTGCGCAATGGCTCATGCGGGCACGAGGGTTCAGCTGTCTCTTACTTTT570.19155800510821347No Hit
ATTGCGCAATGTCTCAGGCGGGCAGTGAAACTGCGAATGGCTCATTAAAT560.18819733835192903No Hit
ATTGCGCAATGGGGGAACGGGGCAAAATAGTGGGAAGATTTATAGGTAGA560.18819733835192903No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT560.18819733835192903No Hit
ATTGCGCAATGGTTCACCCGGGGGAGCCTGAGAAACGGCTACCACATCCA550.18483667159564457No Hit
ATTGCGCAATGTGATAAGGGGGTGAATGCTCAGTATTCCATGAAAATCAG550.18483667159564457No Hit
ATTGCGCAATGATAACAGTGGGGGAGGAATGCGTTCCCTGCCCCCCGTTA550.18483667159564457No Hit
ATTGCGCAATGCACTCTATGGGTGAAAACAACTTTATTGGCTTATTCATG540.18147600483936013No Hit
ATTGCGCAATGAGGATGCGGGGGTGGGAAGATTTATAGGTAGAGGCGACA540.18147600483936013No Hit
ATTGCGCAATGGGTCACGGGGGGTGTGCACCTGTAGTCCCAGCTACTCAG540.18147600483936013No Hit
ATTGCGCAATGGAGTGGCAGGGCACGATCAAAAGGGACAAGCATCAAGCA530.1781153380830757No Hit
ATTGCGCAATGTGCTGCGCGGGTAAGAAAGCGTTCAAGCTCAACACCCAC500.16803333781422236No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCA490.1646726710579379No Hit
ATTGCGCAATGATTATGGTGGGGAAAGCGTTCAAGCTCAACACCCACTAC470.157951337545369No Hit
ATTGCGCAATGTGCTTATGGGGGACTTCAAAGACAATCAAGAAATGTCAC470.157951337545369No Hit
ATTGCGCAATGGCCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCTCTCA470.157951337545369No Hit
ATTGCGCAATGGGAATTCCGGGAAGAGATGAAGCCAAAGCGAAAGCGAGT470.157951337545369No Hit
ATTGCGCAATGCGTACACCGGGATAGTGGGAAGATTTATAGGTAGAGGCG460.15459067078908453No Hit
ATTGCGCAATGATGGAGTCGGGGCAAAATAGTGGGAAGATTTATAGGTAG460.15459067078908453No Hit
ATTGCGCAATGAGGACATCGGGCTACCTAAAAAATCCCAAACATATAACT460.15459067078908453No Hit
ATTGCGCAATGCTGTGTAGGGGGCGGGCGGGCGGTCCGCCGCGAGGCGAG450.1512300040328001No Hit
ATTGCGCAATGCCTTGGTCGGGACGATCAAAAGGGACAAGCATCAAGCAC450.1512300040328001No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG440.14786933727651566No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC440.14786933727651566No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTTCACCACGGATCG430.1445086705202312No Hit
ATTGCGCAATGAGAGATCCGGGTGTCCGGGGCTGCACGCGCGCTACACTG420.14114800376394676No Hit
ATTGCGCAATGATGAGAACGGGGATGTCCGGGGCTGCACGCGCGCTACAC420.14114800376394676No Hit
ATTGCGCAATGAGTAGCATGGGGGGGGGGATGCGTGCATTTATCAGATCA410.13778733700766232No Hit
ATTGCGCAATGCGTATAACGGGCAAAACCAACCCGGTCAGCCCCTCTCCG410.13778733700766232No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC400.13442667025137786No Hit
ATTGCGCAATGTTGTTGCCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGC400.13442667025137786No Hit
ATTGCGCAATGTGCCATGGGGGGGGCAGGACTATGGGAGGCTGCCCTGTA400.13442667025137786No Hit
ATTGCGCAATGGCTGTATCGGGGTGATTTTGAGGGACCTAAGGGACAGTC390.13106600349509342No Hit
ATTGCGCAATGCTTGGAACGGGGCAAAATAGTGGGAAGATTTATAGGTAG390.13106600349509342No Hit
ATTGCGCAATGGTAATTCAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGC380.127705336738809No Hit
ATTGCGCAATGTCTGTGGGGGGATGTTAAACCAGTACGAAATCCAAAACC370.12434466998252454No Hit
ATTGCGCAATGACGCCAGGGGGGATCAAAAGGGACAAGCATCAAGCACGC360.12098400322624009No Hit
ATTGCGCAATGTACGACCGGGGGACAACCTTAGCCAAACCATTTACCCAA360.12098400322624009No Hit
ATTGCGCAATGGGCCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCTCTC350.11762333646995564No Hit
ATTGCGCAATGCTGCTTGGGGGATAGTGGGAAGATTTATAGGTAGAGGCG350.11762333646995564No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA340.11426266971367119No Hit
ATTGCGCAATGATCTCCCGGGGGCGAATGGCTCATTAAATCAGTTATGGT340.11426266971367119No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC340.11426266971367119No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT340.11426266971367119No Hit
ATTGCGCAATGTCGTTGTTGGGTACTTGGATAACTGTGGTAATTCTAGAG330.11090200295738675No Hit
ATTGCGCAATGCACCCTCTGGGGGATAACTGTGGTAATTCTAGAGCTAAT330.11090200295738675No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG320.1075413362011023No Hit
ATTGCGCAATGGATCCCGGGGGCGAATGGCTCATTAAATCAGTTATGGTT320.1075413362011023No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG310.10418066944481785No Hit
ATTGCGCAATGAACGGGGGGGGTGGTATGGAGAGACAACAGGTGATGTTT310.10418066944481785No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG300.10082000268853342No Hit
ATTGCGCAATGCGGGGAATGGGGGGAGAAAGGAGAGAAAGGAAAGCGCGA300.10082000268853342No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)