FastQCFastQC Report
Tue 17 Jan 2023
EGAF00007822604

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822604
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7745
Sequences flagged as poor quality0
Sequence length151
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT5747.411233053582957No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCGATGGTTGTGTA3784.8805681084570685No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC2513.240800516462234No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC470.6068431245965139No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCGATGGTT430.5551969012265978No Hit
CTTATACACATCTGACGCTGCCGACGATTCGATGGTTGTGTAGATCTCGG350.4519044544867657No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCGATGGTTGTG310.40025823111684955No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC310.40025823111684955No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCGA310.40025823111684955No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG280.3615235635894125No Hit
CTCTTATACACATCTGACGCTGCCGACGATTCGATGGTTGTGTAGATCTC250.32278889606197547No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG230.2969657843770174No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT230.2969657843770174No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA230.2969657843770174No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220.28405422853453843No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG220.28405422853453843No Hit
CATCTGACGCTGCCGACGATTCGATGGTTGTGTAGATCTCGGTGGTCGCC190.24531956100710137Illumina RNA PCR Primer (95% over 22bp)
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCGATGGTTGTGTT180.23240800516462234No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC180.23240800516462234No Hit
GATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCGAT180.23240800516462234No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT170.2194964493221433No Hit
ATACACATCTGACGCTGCCGACGATTCGATGGTTGTGTAGATCTCGGTGG170.2194964493221433No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA170.2194964493221433No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC150.19367333763718528No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCGATGGTTGTGGA140.18076178179470626No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA130.16785022595222726No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGA130.16785022595222726No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAT130.16785022595222726No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG120.1549386701097482No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCA120.1549386701097482No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGTCGATGGTTGTGTA120.1549386701097482No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCG120.1549386701097482No Hit
GAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA110.14202711426726922No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGC110.14202711426726922No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAAATGTA100.12911555842479017No Hit
ACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCGA100.12911555842479017No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCGAGGGTTGTGTA100.12911555842479017No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCA100.12911555842479017No Hit
GCAGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGAC100.12911555842479017No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGAAA90.11620400258231117No Hit
CATCTGACGCTGCCGACGATTCGATGGTTGTGTAGATCTCGGGGGTCGCC90.11620400258231117Illumina Single End PCR Primer 1 (95% over 21bp)
GTCTCTTATACACATCTGACGCTGCCGACGATTCGATGGTTGTGTAGATC90.11620400258231117No Hit
CATCTGACGCTGCCGACGATTCGATGGTTGTGTAGATCTCGGTGGGCGCC90.11620400258231117Illumina Single End PCR Primer 1 (95% over 21bp)
CATCTGACGCTGCCGACGATTCGATGGTTGTGTAGATCTCGGTGGTGGCC90.11620400258231117Illumina Single End PCR Primer 1 (95% over 21bp)
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90.11620400258231117No Hit
CATCTGACGCTGCCGACGATTCGATGGTTGTGTAGATCTCGGTGGGGGGC90.11620400258231117No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGA90.11620400258231117No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAA80.10329244673983216No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATCTGTC80.10329244673983216No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCC80.10329244673983216No Hit
CTCTTATACACATCTGACGCTGCCGACGATTCGATGGTTGTGTAGTTCTC80.10329244673983216No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCGATGGGTGTGTA80.10329244673983216No Hit
AGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA80.10329244673983216No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATAT80.10329244673983216No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80.10329244673983216No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA80.10329244673983216No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)