FastQCFastQC Report
Tue 7 May 2024
EGAF00007822605

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822605
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20636
Total Bases3.1 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAACAGGTTAATCG7693.7264973832138013No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC6553.1740647412289205No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAACAGGTTAATCT5682.752471409187827No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC3951.914130645473929No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1830.8867997673967823No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAACAGGTTAAT1080.5233572397751503No Hit
ATTGCGCAATGCCTCTCGGGGGGAATATTGGACAATGGGCGCAAGCCTGA1010.48943593719713124No Hit
ATTGCGCAATGCATTACACGGGGTCTGAAACAACGATTTTAAAGGGAACA920.4458228338825354No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC910.44097693351424694No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG910.44097693351424694No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAACAGGTT900.43613103314595847No Hit
ATTGCGCAATGCGTTTGGTGGGGGAATTTCTATTTTAAGTGAAATTAATT870.42159333204109317No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACGAAC840.40705563093622793No Hit
ATTGCGCAATGATCACCAGGGGCATATCAATAAGCGGAGGAAAAGAAACT790.3828261290947858No Hit
ATTGCGCAATGGCTCACTGGGGTGGAAATGACATCTGTGGGTGTTTGGTT720.3489048265167668No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT710.3440589261484784No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT670.3246753246753247No Hit
ATTGCGCAATGTCACGGATGGGGGTTCCAGGAAATAGCCCCTCCATTATA660.31982942430703626No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAAC540.2616786198875751No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA510.24714091878270983No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG460.2229114169412677No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT460.2229114169412677No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAACA430.2083737158364024No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC420.20352781546811397No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACGAACAGGTTAATCG420.20352781546811397No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC410.19868191509982555No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC370.17929831362667184No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT360.1744524132583834No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACGAA350.16960651289009498No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG330.15991471215351813No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG320.1550688117852297No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGT300.14537701104865283No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC290.14053111068036442No Hit
ATTGCGCAATGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAACA290.14053111068036442No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC270.13083930994378756No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT270.13083930994378756No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG270.13083930994378756No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGTC260.12599340957549912No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTG230.11145570847063385No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTC230.11145570847063385No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCTT220.10660980810234541No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACGAACAGGT220.10660980810234541No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACGAACAGGTTAATCGCG220.10660980810234541No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT210.10176390773405698No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)