FastQCFastQC Report
Tue 17 Jan 2023
EGAF00007822611

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822611
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8231
Sequences flagged as poor quality0
Sequence length151
%GC60

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC4034.8961244077268855No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC2713.292431053334953No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGATGTTCGCATCG1331.6158425464706596No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACCGATGTTCGCATCG670.8139958692746931No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGATGTTCGCATCT520.6317579880937918No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA330.40092333859798324No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG310.3766249544405297No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT300.36447576236180296No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGATGTTCG250.3037298019681691No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAT230.27943141781071557No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGATGTTCGCAT230.27943141781071557No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG220.2672822257319888No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACCGATGTTCGCATCT160.19438707325962823No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGATGTTCGCATGG150.18223788118090148No Hit
ATCTGTGGCTTATACACATCTCCGAGCCCACGAGACCGATGTTCGCATCG130.15793949702344795No Hit
ATTGCGCAATCTGTCTCTTATACACCTGGCTCTTATACACATCTCCGAGC130.15793949702344795No Hit
ATTGCGCAATGTACTGATTGGGGGGGAATAGGGAGAAGCAGAACCGAGGA120.14579030494472117No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT110.1336411128659944No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC100.12149192078726764No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGATGTTCGCAGCG100.12149192078726764No Hit
ATTGCGCAATGCATCTCTAGGGGGGGAATAGGGAGAAGCAGAACCGAGGA100.12149192078726764No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT90.10934272870854088No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCGA90.10934272870854088No Hit
ATTGCGCAATGTATGCTCTGTCTCTTATACACATCTCCGAGCCCACGAGA90.10934272870854088No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGATG90.10934272870854088No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)