FastQCFastQC Report
Tue 7 May 2024
EGAF00007822613

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822613
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13309
Total Bases2 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCGCCATACTATCG6134.6059057780449315No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCGCCATACTATCT5293.9747539259147944No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC3132.351792020437298No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC2541.90848298144113No Hit
ATTGCGCAATGTACTAGTCGGGGGGGGCGCAACGCCCTCGACCTATTCTC1571.1796528664813284No Hit
ATTGCGCAATGCAGCTTTTGGGGGAGGGTCCTTGAGGCCTGACTGCGTAC1411.059433466075588No Hit
ATTGCGCAATGCAGCCGCCGGGTTGAGAGAACTCGGGTGAAGGAACTAGG1130.8490495153655421No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCGCCATAC1110.8340220903148245No Hit
ATTGCGCAATGTATAGTGAGGGGATGAACATGTCCTGTTTTAAAAAACTG1060.7964535276880307No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA960.7213164024344428No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCGCCATACTAT840.6311518521301375No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG790.5935832895033436No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACCCGCCATACTATCG760.5710421519272673No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC680.510932451724397No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCGC660.49590502667367947No Hit
ATTGCGCAATGACGGGAGAGGGTGTGTATGGCGAGATTCTAAGCGTGCTT640.4808776016229619No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT620.46585017657224437No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT520.3907130513186565No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT500.375685626267939No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG470.35314448869186266No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT430.3230896385904275No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACCCGCCATACTATCT370.27800736343827487No Hit
ATTGCGCAATGACATAGACGGGGAATATCAATGGGTTCCGGCTATGGGTT370.27800736343827487No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG330.24795251333683974No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC310.23292508828612218No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG310.23292508828612218No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCGCC300.2254113757607634No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTG300.2254113757607634No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCG280.21038395071004581No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC260.19535652565932826No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA250.1878428131339695No Hit
ATTGCGCAATGCGGTGGCCGGGTGGATGCCTAGGCACTTAGAGCCGAAGA250.1878428131339695No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT240.1803291006086107No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC240.1803291006086107No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC230.17281538808325195No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG230.17281538808325195No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG200.1502742505071756No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGT200.1502742505071756No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCGCCATA190.14276053798181682No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTGT180.13524682545645803No Hit
GGGGTGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG170.12773311293109926No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC170.12773311293109926No Hit
ATTGCGCAATGTATGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGA160.12021940040574047No Hit
ATTGCGCAATGATGTCCCCGGGGATACATAAGATACAATGTAAGCATTAA160.12021940040574047No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCGCCATACTCTCG160.12021940040574047No Hit
ATTGCGCAATGCGGCAACCGGGGGAATGGGTAAGGGGAGAAAAGGAATAG160.12021940040574047No Hit
ATTGCGCAATGGAAACGAGGGGGGCTGATGATTCCCAAGAGCTCATATCG150.1127056878803817No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCGCCATACTAGCG150.1127056878803817No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGC150.1127056878803817No Hit
ATTGCGCAATGGTTGTCACGGGGAGTTTGATCCTGGCTCAGAACGAACGC150.1127056878803817No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCT150.1127056878803817No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGT140.10519197535502291No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCCGCCATACTATCGCG140.10519197535502291No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGCT140.10519197535502291No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT140.10519197535502291No Hit
ATTGCGCAATGATGCGGCGGGGATACATAAGATACAATGTAAGCATTAAT140.10519197535502291No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)