FastQCFastQC Report
Tue 7 May 2024
EGAF00007822614

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822614
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13309
Total Bases2 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT3962.9754301600420767No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACCAACGAATAGTGTA3662.7500187842813135No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACCAACGAATAGTGGT3232.426929145690886No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC3112.3367645953865805No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACCAACGAATAGTGTT3102.3292508828612215No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACCAACGAATAGTGGA1981.4877150800210384No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACCAACGAATA1180.8866180779923359No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGACCAACGAATAGTG820.61612442707942No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACCAAC610.45833646404688555No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC560.42076790142009163No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC550.41325418889473287No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT530.39822676384401534No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT480.3606582012172214No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG350.2629799383875573No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG310.23292508828612218No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACCAACGAATAGTGGG300.2254113757607634No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACCAACGAATAGTGTG300.2254113757607634No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA270.20287023818468702No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACCAA260.19535652565932826No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG250.1878428131339695No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC240.1803291006086107No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG210.15778796303253437No Hit
GGTATAGGTCGCGCGCTTCAGCGCCATCCATTTTCGGGGCTAGTTGATTC210.15778796303253437No Hit
TGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACCAACGAAT190.14276053798181682No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA180.13524682545645803No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAC180.13524682545645803No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGC180.13524682545645803No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA170.12773311293109926No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGA170.12773311293109926No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT170.12773311293109926No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC170.12773311293109926No Hit
GATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACCAACG160.12021940040574047No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGCAA160.12021940040574047No Hit
GCAGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGAC160.12021940040574047No Hit
CTCCTTAGCGGATTTCGACTTCCATGACCACCGTCCTGCTGTCTTAATCG150.1127056878803817No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC150.1127056878803817No Hit
CCTTCAACCTGGTCATGCCTAGATCACCGGGTTTCGGGTCTAATGCAACA150.1127056878803817No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGCA140.10519197535502291No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGA140.10519197535502291No Hit
GTTACACACTCCTTAGCGGATTTCGACTTCCATGACCACCGTCCTGCTGT140.10519197535502291No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)