FastQCFastQC Report
Tue 7 May 2024
EGAF00007822617

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822617
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16390
Total Bases2.4 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATAGGTTGGATCG5513.3618059792556436No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC5033.0689444783404514No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATAGGTTGGATCT3602.1964612568639414No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC2671.6290420988407563No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG980.5979255643685174No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATAGGTTGGAT920.5613178767541184No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTATA850.5186089078706528No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATAGGTTG850.5186089078706528No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA830.5064063453325198No Hit
ATTGCGCAATGAAGGTCGAGGGGGTTGTCCGGGGCTAAGTGTGTGGACTG750.4575960951799878No Hit
ATTGCGCAATGTACAGGTGGGGAAGCATATCAATAAGCGGAGGAAAAGAA740.45149481391092133No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC600.36607687614399026No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT580.35387431360585725No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTATAGGTTGGATCG570.3477730323367907No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT490.2989627821842587No Hit
ATTGCGCAATGCGTGTAGGGGGCACGTCTAAGTGGGAAAGCATGTGGGAA480.2928615009151922No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT460.2806589383770592No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG420.25625381330079317No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC320.19524100061012814No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG320.19524100061012814No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA310.18913971934106164No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC310.18913971934106164No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG290.17693715680292862No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC280.17083587553386212No Hit
ATTGCGCAATGAATAGCACGGGGGGAGTACGGTCGCAAGATTAAAACTCA280.17083587553386212No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT270.16473459426479561No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGT260.1586333129957291No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAT260.1586333129957291No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGTC260.1586333129957291No Hit
ATTGCGCAATGTCGAGCACGGGGGGAGTACGGTCGCAAGATTAAAACTCA260.1586333129957291No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT240.1464307504575961No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG240.1464307504575961No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGT220.1342281879194631No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTG220.1342281879194631No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATAGGGTGGATCG220.1342281879194631No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATAG210.12812690665039658No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATAGGGTGGATCT210.12812690665039658No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC200.12202562538133008No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATAGGTTGGAGCG200.12202562538133008No Hit
ATTGCGCAATGCGCAGCTTGGGGGGCGCACGGAGGATGCCTTGGCACTAG200.12202562538133008No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACTATAGGTT190.11592434411226357No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATAGGTTGGATCG190.11592434411226357No Hit
ATTGCGCAATGGGAAGGGTGGGAAGTACAGCCAGGTTCTGGCCAACGGTC190.11592434411226357No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC190.11592434411226357No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTATAGGTTGGATCT180.10982306284319707No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTATAGGTTGGATCGCG170.10372178157413056No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGTCT170.10372178157413056No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTGT170.10372178157413056No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)