FastQCFastQC Report
Tue 7 May 2024
EGAF00007822623

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822623
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16533
Total Bases2.4 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCGCTCGTCATCG6483.91943385955362No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC6463.9073368414685783No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCGCTCGTCATCT4892.9577209217927782No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC3802.298433436157987No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1881.1371196999939515No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG1030.622996431379665No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCGCTCGTCAT860.5201717776568077No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCGCTCGT850.5141232686142866No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCG700.42339563297647126No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT700.42339563297647126No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT660.39920159680638717No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT620.3750075606363032No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC550.332667997338656No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCGC520.314522470211093No Hit
ATTGCGCAATGTATCGGACGGGTGAAGACGCTTGCTGGTGGTGTTGTCAT490.2963769430835299No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT470.28427992499848787No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG470.28427992499848787No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC450.2721829069134458No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC450.2721829069134458No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC430.26008588882840383No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC430.26008588882840383No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA380.22984334361579872No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG380.22984334361579872No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC360.21774632553075668No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG360.21774632553075668No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTTCGCTCGTCATCG350.21169781648823563No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG340.2056493074457146No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCGCTCG290.17540676223310953No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCGCTCGTCATCGCG280.1693582531905885No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTC270.16330974414806748No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT260.1572612351055465No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG250.15121272606302547No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT240.14516421702050444No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGT230.13911570797798342No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCGCTCGTCAGCG220.1330671989354624No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTC200.12097018085042038No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCG190.11492167180789936No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTGT190.11492167180789936No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTGT180.10887316276537834No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCGCTCGTCAGCT170.1028246537228573No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGT170.1028246537228573No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)