FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007822625

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822625
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17532
Sequences flagged as poor quality0
Sequence length151
%GC58

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCACGGTACAATCG7844.471822952315765No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCACGGTACAATCT5192.9603011635865846No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC3902.2245037645448322No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC2501.4259639516313027No Hit
ATTGCGCAATGACGGGCAAGGGGAATCTTAGTTCAACTTTAAATTTGCCC2501.4259639516313027No Hit
ATTGCGCAATGAACTTCGGGGGACGGAGGGCCCGACCCGGGTTGGGGGGT1330.758612822267853No Hit
ATTGCGCAATGCGTGTAATGGGGCTGATCTTCCCCAAGAGTCCATATCGA1160.6616472735569244No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCACGGTAC1060.6046087154916724No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC1030.5874971480720967No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1030.5874971480720967No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG910.5190508783937943No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCACGGTACAAT870.49623545516769335No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT830.47342003194159255No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT810.4620123203285421No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACGCAC720.41067761806981523No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT650.3707506274241387No Hit
ATTGCGCAATGTTTGTCTGGGGTCACAGGCATCATCGCAGAGACGGTCTG640.3650467716176135No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT520.296600501939311No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACGCACGGTACAATCG480.2737850787132101No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC460.2623773671001597No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG440.25096965548710926No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA370.21104266484143283No Hit
ATTGCGCAATGGGATCTTGGGGATTCGGTATCAGTGTTATCGCAGATGGT350.1996349532283824No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCACG350.1996349532283824No Hit
ATTGCGCAATGATTGGTACGGGGAAAGAGAATGTATATAGATGTGAGAAA340.19393109742185718No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG340.19393109742185718No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC330.18822724161533197No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC310.17681953000228154No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG310.17681953000228154No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTG310.17681953000228154No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCACGGTACAAGCG300.17111567419575632No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC280.15970796258270592No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGT280.15970796258270592No Hit
ATTGCGCAATGGGATTAAGGGGGAGAATCACTCCCGTCGAAACCCTAAGG250.14259639516313027No Hit
ATTGCGCAATGCTCCCGTTGGGAAGGGCGCACGGTGGATGCCTTGGCACT250.14259639516313027No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACGCA240.13689253935660506No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACGCACGGTACAATCGCG240.13689253935660506No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT240.13689253935660506No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTC230.13118868355007984No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACGCACGGTACAATCT220.12548482774355463No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTGA220.12548482774355463No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC220.12548482774355463No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG210.11978097193702943No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCT210.11978097193702943No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTG200.11407711613050422No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT200.11407711613050422No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCACGGTACAAGCT200.11407711613050422No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGT190.10837326032397901No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGTCT180.10266940451745381No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCACGGTACAATGG180.10266940451745381No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)