FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007822626

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822626
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17532
Sequences flagged as poor quality0
Sequence length151
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCGATGGTTGTGTA11476.542322610084417No Hit
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT4992.8462240474560803No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC3141.791010723248916No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCGATGGTT990.5646817248459959No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCGATGGTTGTG760.43349304129591604No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCGA650.3707506274241387No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT650.3707506274241387No Hit
CTTATACACATCTGACGCTGCCGACGATTCGATGGTTGTGTAGATCTCGG560.31941592516541184No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC510.29089664613278576No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT510.29089664613278576No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCGATGGTTGTGTT500.28519279032626055No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCGATGGTTGTGGA500.28519279032626055No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA480.2737850787132101No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG440.25096965548710926No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCGAGGGTTGTGTA430.24526579968058407No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG400.22815423226100845No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG390.22245037645448323No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG380.21674652064795802No Hit
CTCTTATACACATCTGACGCTGCCGACGATTCGATGGTTGTGTAGATCTC340.19393109742185718No Hit
CATCTGACGCTGCCGACGATTCGATGGTTGTGTAGATCTCGGTGGTCGCC340.19393109742185718Illumina RNA PCR Primer (95% over 22bp)
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC310.17681953000228154No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA300.17111567419575632No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA290.1654118183892311No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC290.1654118183892311No Hit
CTTATACACATCTGACGCTGCCGACGATTCGATGGTTGTGTAGTTCTCGG270.1540041067761807No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCGATGGGTGTGTA260.1483002509696555No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT260.1483002509696555No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCG250.14259639516313027No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC240.13689253935660506No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGATTCGATGGTTGTGTAGA240.13689253935660506No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCA230.13118868355007984No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC230.13118868355007984No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCA230.13118868355007984No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGCA220.12548482774355463No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGTCGATGGTTGTGTA220.12548482774355463No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAA200.11407711613050422No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAT200.11407711613050422No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGC200.11407711613050422No Hit
GTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGA190.10837326032397901No Hit
ATACACATCTGACGCTGCCGACGATTCGATGGTTGTGTAGATCTCGGTGG190.10837326032397901No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATATGT190.10837326032397901No Hit
ATCTATCTCTTATACACATCTGACGCTGCCGACGATTCGATGGTTGTGTA180.10266940451745381No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)