FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007822627

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822627
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17392
Sequences flagged as poor quality0
Sequence length151
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACATGGACGATCG7724.438822447102115No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC5062.909383624655014No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACATGGACGATCT4732.7196412143514257No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC3582.058417663293468No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG1130.6497240110395583No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1120.6439742410303588No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACATGGAC940.5404783808647654No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC940.5404783808647654No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACATGGACGAT890.5117295308187673No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT700.40248390064397427No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTACA660.37948482060717575No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT620.3564857405703772No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT620.3564857405703772No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT510.2932382704691812No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACATGGACGAGCG480.27598896044158233No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG450.25873965041398345No Hit
ATTGCGCAATGAATACTGAGGGGTCATCCGCCGGGACTATCTCCATTACA450.25873965041398345No Hit
AGTCTGCATCACCACCGGATCAACTTCGACAAATACCACCCAGGCTACTT440.25298988040478376No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACAT430.24724011039558416No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA410.23574057037718493No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC410.23574057037718493No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTACATGGACGATCG380.21849126034958602No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG360.20699172033118673No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG290.1667433302667893No Hit
ATTGCGCAATGAAGAAGGGGGGAAATGGAGGAGATAGTCGAAGACCAAAA290.1667433302667893No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACA280.1609935602575897No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG270.15524379024839005No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACTACATGGA270.15524379024839005No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC260.14949402023919045No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGTC260.14949402023919045No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC260.14949402023919045No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC250.1437442502299908No Hit
ATTGCGCAATGAAACGCCGGGGCCGTGGGCGTTGGATACTTGACGGAGCC250.1437442502299908No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGT240.13799448022079117No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACATGGACGAGCT230.13224471021159154No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT220.12649494020239188No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT220.12649494020239188No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTG220.12649494020239188No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGG200.11499540018399264No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCT200.11499540018399264No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGT190.10924563017479301No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAC190.10924563017479301No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTCT190.10924563017479301No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)