FastQCFastQC Report
Tue 7 May 2024
EGAF00007822632

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822632
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14210
Total Bases2.1 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT6574.623504574243491No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC4232.976776917663617No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCTTGTGTTGTGGG1691.1893033075299086No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCGTGTGTTGTGGG1481.0415200562983813No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCTTGTGTTGTGGT990.6966924700914849No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT880.6192821956368755No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT600.422237860661506No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCGTGTGTTGTGGT540.38001407459535536No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG540.38001407459535536No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC480.3377902885292048No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCTTGTGTTGTGTG470.33075299085151305No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCTTGTGTTGTGTT450.3166783954961295No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG430.30260380014074595No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG410.2885292047853624No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC390.2744546094299789No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCTTGTGTTGTGTA390.2744546094299789No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC350.24630541871921183No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCTTGTGGTGTGGG330.23223082336382828No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCGTGTGGTGTGGG330.23223082336382828No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG320.22519352568613651No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT320.22519352568613651No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCTTGTGTT310.21815622800844475No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCGTGTGTTGTGTG300.211118930330753No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCTTGTGTTGTG290.20408163265306123No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCTT280.19704433497536944No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCGTGGGTTGTGGG250.17593244194229415No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA240.1688951442646024No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCGTGTGTT240.1688951442646024No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC230.1618578465869106No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCTTGGGTTGTGGG230.1618578465869106No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA230.1618578465869106No Hit
CGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210.1477832512315271No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAC200.14074595355383532No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190.13370865587614356No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCGTGTGTTGGGGG180.1266713581984518No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCA180.1266713581984518No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA160.11259676284306826No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAA150.1055594651653765No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAA150.1055594651653765No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)