FastQCFastQC Report
Tue 7 May 2024
EGAF00007822637

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822637
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14289
Total Bases2.1 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCAGCTATCG5734.010077682133109No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC5633.9400937784309606No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCAGCTATCT3362.35145916439219No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC3022.113513891804885No Hit
ATTGCGCAATGAGAATGCCGGGCGTGGTGGTGCATGCCTGTAATCCCAGC2621.8335782769962907No Hit
ATTGCGCAATGGGGATGGTGGGGAACCAGGAACGCATACCACACCACAAT1180.8258100636853524No Hit
ATTGCGCAATGAGAACTGAGGGGTGAGGGAAAGGCGAAAAGAACCCCGGG1040.7278325985023444No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA990.6928406466512702No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCAGC840.5878647910980475No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCAGCTAT630.4408985933235356No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG600.41990342221289106No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCA530.3709146896213871No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC520.3639162992511722No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG440.30792917628945343No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT420.29393239554902373No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT410.28693400517880885No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTTCACCAGCTATCG400.279935614808594No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT390.2729372244383792No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC390.2729372244383792No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT290.20295332073623065No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA290.20295332073623065No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCAC290.20295332073623065No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC270.18895653999580095No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCAGCGATCG250.17495975925537124No Hit
ATTGCGCAATGTAACGTATGGGCACTAGAAGCCGAAGAAGGACGTTACTA210.14696619777451186No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG210.14696619777451186No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC200.139967807404297No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG190.13296941703408216No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCAGCTAGCG180.1259710266638673No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTC170.11897263629365247No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT170.11897263629365247No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG170.11897263629365247No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG170.11897263629365247No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTTCACCAGCTATCT160.11197424592343762No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCAG160.11197424592343762No Hit
ATTGCGCAATGGGCATAGGGGGCAGATTTCGATGTGGAAAAACATGATCA150.10497585555322277No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCAGCTATGG150.10497585555322277No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC150.10497585555322277No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)