FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007822643

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822643
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17274
Sequences flagged as poor quality0
Sequence length151
%GC57

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAACAGGTTAATCG6403.704990158619891No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAACAGGTTAATCT4592.657172629385203No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC4222.4429778858399906No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC2821.6325112886418895No Hit
ATTGCGCAATAAGGCGGTGGGGGCCTTTCGCGCTACCCGGGGACGGGTCC2511.4530508278337386No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1190.688896607618386No Hit
ATTGCGCAATGTGTAGGACGGGCGGAGCATGTGGATTAATTCGATGCAAC1100.6367951835127938No Hit
ATTGCGCAATGCACTAGAAGGGGCACGGCGGTGGACGACGACCCTCTTGA1050.6078499478985759No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG930.538381382424453No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAACAGGTT830.48049091119601717No Hit
ATTGCGCAATGTCTGGTCGGGGAAAGGGTGGTAAAGGTCTCGGCAAGGGT810.46891281695033No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAACAGGTTAAT790.45733472270464276No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC770.44575662845895564No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACGAAC640.37049901586198913No Hit
ATTGCGCAATGATGCAGTAGGGCAAGGATGCTGCTCTCATGCGCGCTGTC640.37049901586198913No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT520.30103045038786613No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT520.30103045038786613No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT510.2952414032650226No Hit
ATTGCGCAATGGTAGGCAGGGATGAGGCCCGGGGTGGGGGTGGCAGCGGT490.28366330901933545No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA460.26629616765080466No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAACA410.23735093203658678No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG400.2315618849137432No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACGAACAGGTTAATCG340.19682760217668172No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT320.18524950793099457No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG320.18524950793099457No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC310.17946046080815098No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGT290.1678823665624638No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACGAA270.15630427231677665No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC260.15051522519393307No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG250.1447261780710895No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG250.1447261780710895No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACGAACAGGTTAATCGCG250.1447261780710895No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAACAGGGTAATCG240.13893713094824592No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGTC240.13893713094824592No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGTCT220.12735903670255877No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAACAGGTTAATGG220.12735903670255877No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT220.12735903670255877No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAAC220.12735903670255877No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG210.12156998957971517No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC200.1157809424568716No Hit
ATTGCGCAATGGCCTGGTTGGGAAGTTAATAAGGGCGCACGGTGGATGCC200.1157809424568716No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT190.10999189533402803No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACGAACAGGTTAATCT190.10999189533402803No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACGAACAGGT180.10420284821118443No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)