FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007822645

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822645
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14759
Sequences flagged as poor quality0
Sequence length151
%GC59

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGCGCTTAGAATCG5863.970458703164171No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC4713.1912731214851955No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGCGCTTAGAATCT3772.554373602547598No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC2881.951351717596043No Hit
ATTGCGCAATGCACAAGGTGGGATCTCAATAAAGATGGAGAGGTGCCCCC1350.9146961176231452No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA950.6436750457348058No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG860.5826953045599296No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGCGCTTAG850.5759197777627211No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGCGCTTAGAAT790.5352666169794702No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT700.47428687580459383No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACCGCGCTTAGAATCG690.4675113490073853No Hit
ATTGCGCAATGAGCAAAGTGGGGGCGCATGCGCATTGCGTCCCTTCCTCT690.4675113490073853No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC650.4404092418185514No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT640.43363371502134296No Hit
ATTGCGCAATGTATACGAAGGGGGATCAAGCGACTAAGTGCATATGGTGG610.41330713462971747No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCGCG600.40653160783250897No Hit
ATTGCGCAATGTGCGACATGGGCGAGATGTTCCACAAGGAGCTCGACCGC480.3252252862660072No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG460.3116742326715902No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT400.2710210718883393No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGCGC390.26424554509113085No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG390.26424554509113085No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC370.25069449149671386No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC360.2439189646995054No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG360.2439189646995054No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA330.22359238430787995No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT300.20326580391625448No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCGC290.19649027711904601No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC290.19649027711904601No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG280.18971475032183752No Hit
ATTGCGCAATGCCGTCCCGGGGGTAAGAGCGCATGGTGGATGCCTTGGCA270.18293922352462905No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCGCGCTTAGAATCGCG260.17616369672742055No Hit
ATTGCGCAATGAGGAGATGGGGAGGATAGGCCGGAGGCAGAGAAGCGGGT260.17616369672742055No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACCGCGCTTAGAATCT250.16938816993021208No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGCG250.16938816993021208No Hit
ATTGCGCAATGTGTATACTGGGGTGGGTGAGTGAGTGATCCTGTACTGTC230.1558371163357951No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG230.1558371163357951No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCT220.14906158953858661No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGCT220.14906158953858661No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC210.14228606274137814No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGT200.13551053594416965No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT190.12873500914696118No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGCGCTTAGACTCG180.1219594823497527No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTG170.11518395555254421No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTGT170.11518395555254421No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTGT170.11518395555254421No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGCGCTTAGAAGCG160.10840842875533574No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGC160.10840842875533574No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGTC160.10840842875533574No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACCGCGCTTA160.10840842875533574No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCCT160.10840842875533574No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)