FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007822649

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822649
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10925
Sequences flagged as poor quality0
Sequence length151
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGATGTTCGCATCG5024.5949656750572085No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGATGTTCGCATCT3062.80091533180778No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC2352.1510297482837526No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC1891.7299771167048055No Hit
ATTGCGCAATGGCTGAGGGGGGAGAATCAGTTGAGGTCAGGAGTTCAAGA1181.080091533180778No Hit
ATTGCGCAATGGCACACCGGGGAATGGGAAGCGCCACCACCAGCTCAGAA860.7871853546910754No Hit
ATTGCGCAATGTCGGTTCGGGGGGTTCCAGGAAATAGCCCCTCCATTATA840.7688787185354691No Hit
ATTGCGCAATGGACTATACGGGGAGGGTCCTTGAGGCCTGACTGCGTACC800.7322654462242564No Hit
ATTGCGCAATGTAGACGAGGGGAGCCTGAACGGCACTGGTGGCGATAGCG590.540045766590389No Hit
ATTGCGCAATGCCTTCATCGGGATAGGGAACGTGAGCTGGGTTTAGACCG570.5217391304347827No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGATGTTCGCAT570.5217391304347827No Hit
ATTGCGCAATGTTACCCGGGGGGGTTGCTGAGGGGCGCTCGAAAGCGAGC550.5034324942791762No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGATGTTCG530.48512585812356984No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA530.48512585812356984No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG470.4302059496567505No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAT390.35697940503432496No Hit
ATTGCGCAATGCGAATGTGGGGGCCCCTCGACCACTCCTTTGGCGCTTCG390.35697940503432496No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC380.34782608695652173No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACCGATGTTCGCATCG340.3112128146453089No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT340.3112128146453089No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT340.3112128146453089No Hit
ATTGCGCAATGGACTTCCCGGGCACGTACAAGGCTGGCGTGGAGCTCGAT330.30205949656750575No Hit
ATTGCGCAATGCTTGGAACGGGGATGGCTCCAAGAAGGCCTTCGCCCGTC310.28375286041189934No Hit
ATTGCGCAATTCTCCTACGGGATGACCAATCAAAAGTGACTTATAATAAT240.2196796338672769No Hit
ATTGCGCAATGCTGCTTGTGGGTGGTAGGGGAGCGTTCTTACTTCAGGGA240.2196796338672769No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGATGTTCGCAGCG200.1830663615560641No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG190.17391304347826086No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC190.17391304347826086No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA180.16475972540045766No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT170.15560640732265446No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC170.15560640732265446No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT170.15560640732265446No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT160.14645308924485126No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG160.14645308924485126No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG150.13729977116704806No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC150.13729977116704806No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC150.13729977116704806No Hit
ATTGCGCAATGTGAGCCATGGGAACTTGCCCACGCTCATCACCACGTGCT150.13729977116704806No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCGA140.12814645308924486No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGCT140.12814645308924486No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT140.12814645308924486No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG140.12814645308924486No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTGT140.12814645308924486No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG140.12814645308924486No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACCGATGTTC130.11899313501144164No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC130.11899313501144164No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGATG130.11899313501144164No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCGATGTTCGCATCGCG120.10983981693363845No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCT120.10983981693363845No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTC110.10068649885583525No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGTC110.10068649885583525No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGG110.10068649885583525No Hit
ATTGCGCAATGTAGCTCTTGGGTGTGTGGGTGGTTTGTGTGTTCGTTATT110.10068649885583525No Hit
ATTGCGCAATGCCGAGGGTGGGGGGTAGTGGATGGACAACGGGTTAATAT110.10068649885583525No Hit
ATTGCGCAATGTACGGATAGGGGTAGATCCAAGCTTCCAACTCTCCGAAA110.10068649885583525No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)