FastQCFastQC Report
Tue 7 May 2024
EGAF00007822661

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822661
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11061
Total Bases1.6 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC60

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC4003.6163095560980016No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC2242.025133351414881No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCACGGTACAATCG2041.844317873609981No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCACGGTACAATCT1060.9583220323659705No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACGCACGGTACAATCG1020.9221589368049905No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCACGGTACAAT520.4701202422927403No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCACGGTAC480.4339571467317603No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACGCACGGTACAATCT460.4158755989512702No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG390.3525901817195552No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC380.3435494078293102No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACGCAC370.3345086339390652No Hit
ATTGCGCAATGCAGGAGAGGGGAAAGAATGTGAGATTGGATTGTGTGTGT350.3164270861585752No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG330.29834553837808514No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA300.27122321670735017No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCACGGTACAAGCG270.24410089503661514No Hit
CCTGTAGTTTGAGCCCAAGGAGTTTAATTCCAAATATCATGATTTTAACT230.2079377994756351No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT230.2079377994756351No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC210.18985625169514508No Hit
ATTGCGCAATGGTACCCCTGGGAAGGTGACAGCATTGCTTCTGTGTAAAT190.1717747039146551No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT180.16273393002441008No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT180.16273393002441008No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT170.15369315613416507No Hit
ATCTGTGGCTTATACACATCTCCGAGCCCACGAGACGCACGGTACAATCG150.13561160835367508No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA150.13561160835367508No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCACGGTACAATGG150.13561160835367508No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG150.13561160835367508No Hit
ATTGCGCAATGTGCCTTTGGGGGTAGAGCGTCCAGCGAATACCCCCGGGG140.12657083446343007No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG140.12657083446343007No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG140.12657083446343007No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGT130.11753006057318507No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC130.11753006057318507No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT120.10848928668294007No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT120.10848928668294007No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG120.10848928668294007No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTGT120.10848928668294007No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)