FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007822665

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822665
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13553
Sequences flagged as poor quality0
Sequence length151
%GC58

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACATGGACGATCG6795.0099608942669525No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACATGGACGATCT4393.2391352468088246No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC2952.1766398583339486No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC2141.5789862023168302No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACATGGACGAT860.634545857005829No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG840.6197889766103446No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACATGGAC700.5164908138419538No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT630.46484173245775845No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC620.45746329226001625No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTACA600.4427064118645318No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT570.4205710912713052No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTACATGGACGATCG530.3910573304803365No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC510.3763004500848521No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA510.3763004500848521No Hit
ATTGCGCAATGAACTAGCGGGGGCGAGCGAAATCGGAGAAGCCTGTTAGT440.3246513687006567No Hit
ATTGCGCAATGTGAGTGTCGGGGACAGTTGACACCAAGGGATCTTGAAGT420.3098944883051723No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT390.2877591677119457No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACATGGACGAGCG380.28038072751420345No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA330.24348852652549252No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC320.2361100863277503No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT310.22873164613000813No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG290.21397476573452373No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG250.18446100494355494No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG240.17708256474581274No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAC220.16232568435032835No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACAT220.16232568435032835No Hit
ATTGCGCAATGGCATTATAGGGGACTTAACTTTGACGACCTCCCAGATGA220.16232568435032835No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC220.16232568435032835No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG210.15494724415258615No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC190.14019036375710173No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACATGGACGATGG190.14019036375710173No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT180.13281192355935953No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGTC180.13281192355935953No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC180.13281192355935953No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGT170.12543348336161733No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACA170.12543348336161733No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT160.11805504316387515No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACTACATGGA160.11805504316387515No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTGT150.11067660296613295No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG150.11067660296613295No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCT150.11067660296613295No Hit
GGGGGCTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG140.10329816276839075No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTACATGGACGATCT140.10329816276839075No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTG140.10329816276839075No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTCT140.10329816276839075No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)