FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007822667

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822667
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13158
Sequences flagged as poor quality0
Sequence length151
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCGATAACTATCG5414.111567107463141No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC5324.043167654658763No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC3522.675178598571211No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCGATAACTATCT3362.553579571363429No Hit
ATTGCGCAATGGAATCCCAGGGGATGAGAATATTGCCGATGATGCTAAGA1691.284389724882201No Hit
ATTGCGCAATGTTGGCGTGGGGATCCCGTCCATCAAGTGGTGCGGAGCTG1571.1931904544763643No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1170.8891928864569083No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCGATAAC840.6383948928408573No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG620.47119623043015657No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCGATAACTAT580.44079647362821095No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC580.44079647362821095No Hit
ATTGCGCAATGGGGGGATCGGGTAAGTAAAGGGAAGGAAAAGAAGAGTAA570.43319653442772454No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCG520.3951968384252926No Hit
ATTGCGCAATGACGGCTGGGGGGTGATGGTGAGCCACCGCTCTGGGGAGA500.37999696002431976No Hit
ATTGCGCAATGGGGGGCTCGGGTAAGTAAAGGGAAGGAAAAGAAGAGTAA460.34959720322237425No Hit
ATTGCGCAATGCTCACTAGGGGAGGGAGAGCGATTTATCCTTCGTGGGTA430.32679738562091504No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT430.32679738562091504No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCGA410.3115975072199422No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTCCGATAACTATCG400.3039975680194559No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG390.29639762881896947No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT380.288797689618483No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA310.23559811521507829No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC270.2051983584131327No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT260.1975984192126463No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT240.1823985408116735No Hit
ATTGCGCAATGCCGGGTTCGGGAGTTCCAGACCAGCCTGACCAACATGGA220.1671986624107007No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG210.15959872321021432No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC200.15199878400972794No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTG200.15199878400972794No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT190.1443988448092415No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTCCGATAACTATCT180.13679890560875513No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG180.13679890560875513No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCC170.12919896640826875No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC170.12919896640826875No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCG160.12159902720778233No Hit
ATTGCGCAATGAGGGCAGGGGGGGGAGCCGCGGCTTATGGTGCAGACATG150.11399908800729594No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG150.11399908800729594No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGT140.10639914880680955No Hit
GGGGGCTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG140.10639914880680955No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCGATAACTATGT140.10639914880680955No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCT140.10639914880680955No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)