FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007822672

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822672
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12651
Sequences flagged as poor quality0
Sequence length151
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAAAGTACGAGAGTGTA9667.635760018970833No Hit
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT5284.173583115959213No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC3572.821911311358786No Hit
CATCTGACGCTGCCGACGAAAGTACGAGAGTGTAGATCTCGGTGGTCGCC1741.3753853450320133Illumina Single End PCR Primer 1 (96% over 25bp)
CTTATACACATCTGACGCTGCCGACGAAAGTACGAGAGTGTAGATCTCGG1641.2963402102600585No Hit
CTCTTATACACATCTGACGCTGCCGACGAAAGTACGAGAGTGTAGATCTC1010.7983558611967433No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAAAGTACGAGA750.5928385107896609No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGAAAGTACGAGAGTG660.5216978894949016No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAAAGTA630.4979843490633152No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT570.45055726820014225No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT570.45055726820014225No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG490.38732116038257847No Hit
ATACACATCTGACGCTGCCGACGAAAGTACGAGAGTGTAGATCTCGGTGG480.379416646905383No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC460.363607619950992No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC440.3477985929966011No Hit
CATCTGACGCTGCCGACGAAAGTACGAGAGTGTAGATCTCGGTGGGCGCC390.30827602561062367No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA360.28456248517903726No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG310.24503991779305984No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGAAAGTACGAGAGTGTAGA310.24503991779305984No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC300.23713540431586438No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAA270.21342186388427792No Hit
ACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAAAGTA260.20551735040708247No Hit
CATCTGACGCTGCCGACGAAAGTACGAGAGTGTAGATCTCGGGGGTCGCC250.197612836929887No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG250.197612836929887No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC230.181803809975496No Hit
GTCTCTTATACACATCTGACGCTGCCGACGAAAGTACGAGAGTGTAGATC230.181803809975496No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC230.181803809975496No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG220.17389929649830055No Hit
GCATATGGAGAGGACTCATCTCGGTCAGCCTTCACCTTCATCCCACCAGT220.17389929649830055No Hit
TGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAAAGTACGAG210.16599478302110504No Hit
CATCTGACGCTGCCGACGAAAGTACGAGAGTGTAGATCTCGGTGGTCGGC210.16599478302110504Illumina Single End PCR Primer 1 (95% over 23bp)
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATAT210.16599478302110504No Hit
CTTATACACATCTGACGCTGCCGACGAAAGTACGAGAGTGTAGTTCTCGG210.16599478302110504No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAAAGT210.16599478302110504No Hit
CATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAAAGTAC190.15018575606671408No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAA190.15018575606671408No Hit
GATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAAAGTAC190.15018575606671408No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGA180.14228124258951863No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAA170.13437672911232312No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT170.13437672911232312No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA160.12647221563512764No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCA160.12647221563512764No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160.12647221563512764No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA150.11856770215793219No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCG150.11856770215793219No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAAA140.1106631886807367No Hit
CATCTGACGCTGCCGACGAAAGTACGAGAGTGTAGATCTCGGTGGTCGCG140.1106631886807367Illumina Single End PCR Primer 1 (95% over 24bp)
CGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140.1106631886807367No Hit
CATCCTGGGCAGCCAACATGGCTGCATATGGAGAGGACTCATCTCGGTCA130.10275867520354123No Hit
CATATGGAGAGGACTCATCTCGGTCAGCCTTCACCTTCATCCCACCAGTC130.10275867520354123No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGC130.10275867520354123No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130.10275867520354123No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)