FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007822673

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822673
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10320
Sequences flagged as poor quality0
Sequence length151
%GC59

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCATCGGCATCG4804.651162790697675No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC3903.77906976744186No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCATCGGCATCT2862.7713178294573644No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC2232.1608527131782944No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC770.7461240310077519No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCATCGG760.7364341085271318No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG750.7267441860465116No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA720.6976744186046512No Hit
ATTGCGCAATGGGAGGATGGGGGGGCATAAACCAAGGGAGGCGCAGCAGC650.6298449612403101No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT600.5813953488372093No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT580.562015503875969No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT580.562015503875969No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACAACCATCGGCATCG520.5038759689922481No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT500.4844961240310077No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACAACC420.4069767441860465No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCATCGGCAT420.4069767441860465No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG380.3682170542635659No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC350.3391472868217054No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG300.29069767441860467No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCATCGGCAGCG290.2810077519379845No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC270.2616279069767442No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG270.2616279069767442No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG260.25193798449612403No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTC250.24224806201550386No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA240.23255813953488372No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACAAC240.23255813953488372No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC230.22286821705426357No Hit
ATTGCGCAATGGAGAGTACGGGGACGCCATGCACGCCGCACGCCGCACGG210.20348837209302326No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCA200.1937984496124031No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC200.1937984496124031No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCATCG190.18410852713178294No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC180.1744186046511628No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACAACCATCGGCATCT180.1744186046511628No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTG160.15503875968992248No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT160.15503875968992248No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTG160.15503875968992248No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTGT160.15503875968992248No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCATCGGCATCGCG150.14534883720930233No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGT150.14534883720930233No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTCT130.12596899224806202No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTGT130.12596899224806202No Hit
ATTGCGCAATGCGGCTAATGGGATAAAAAGAGAAAATAAAAAAAAAATAA130.12596899224806202No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG130.12596899224806202No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTG120.11627906976744186No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGT110.1065891472868217No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTG110.1065891472868217No Hit
ATTGCGCAATGTATGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGA110.1065891472868217No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGCT110.1065891472868217No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCATCGGCATCTCG110.1065891472868217No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGTC110.1065891472868217No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCCT110.1065891472868217No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)