FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007822679

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822679
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15018
Sequences flagged as poor quality0
Sequence length151
%GC58

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAACAGGTTAATCG6914.601145292315888No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC4823.2094819549873486No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAACAGGTTAATCT4162.770009322146757No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC2671.7778665601278467No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAACAGGTTAAT900.5992808629644426No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACGAAC810.5393527766679984No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAACAGGTT690.4594486616060727No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA680.4527899853509122No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG630.4194966040751098No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC500.3329338127580237No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT460.3062991077373818No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACGAACAGGTTAATCG450.2996404314822213No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT440.2929817552270608No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG350.23305366893061658No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACGAA310.2064189639099747No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC300.1997602876548142No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC280.18644293514449328No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA280.18644293514449328No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG280.18644293514449328No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT270.1797842588893328No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT270.1797842588893328No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC270.1797842588893328No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAAC270.1797842588893328No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT250.16646690637901185No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGT240.15980823012385137No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAACAGGTTAAGCG240.15980823012385137No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC240.15980823012385137No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAACA230.1531495538686909No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC220.1464908776135304No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACGAACAGGTTAATCT210.13983220135836996No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG210.13983220135836996No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACGAACAGGTTAATCGCG210.13983220135836996No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG200.13317352510320948No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTG190.126514848848049No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGTC190.126514848848049No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG180.11985617259288853No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACGAACAGGT180.11985617259288853No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAACAGGGTAATCG170.11319749633772805No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAACAGGGTAATCT170.11319749633772805No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT170.11319749633772805No Hit
ATTGCGCAATGCGATTGGGGGTGAATTACTTGCATCAGATAGAATATTTA160.10653882008256757No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)