FastQCFastQC Report
Tue 7 May 2024
EGAF00007822681

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822681
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13402
Total Bases2 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC60

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGCGCTTAGAATCG6324.715714072526488No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGCGCTTAGAATCT4733.529323981495299No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC3162.357857036263244No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC2221.6564691837039247No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGCGCTTAG1000.7461572899567228No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG820.6118489777645127No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCGCG780.5820026861662438No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC760.5670795403671094No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGCGCTTAGAAT740.5521563945679749No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT730.5446948216684077No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA710.5297716758692733No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT630.47007909267273545No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT610.45515594687360095No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACCGCGCTTAGAATCG590.4402328010744665No Hit
ATTGCGCAATGAACCCCGCGGGGGGTGCTTTGCAAGGCCTGTTTAAGGGA570.42530965527533204No Hit
ATTGCGCAATGAGCCTAGAGGGGGGGCGCGATGGGGAGCGAAACTAAGTA520.38800179077749586No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGCGC380.2835397701835547No Hit
ATTGCGCAATGCGACGAAAGGGCGGCACATTATCGTTAGATGAATACATA380.2835397701835547No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG380.2835397701835547No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA370.27607819728398747No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG370.27607819728398747No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT360.26861662438442024No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC360.26861662438442024No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG360.26861662438442024No Hit
ATTGCGCAGTGGCGTCGACGTATTACTTACAGAACAAAGGATCGTGCAGC330.24623190568571854No Hit
ATTGCGCAATGTCTGGCACGGGGGTCGTTCAAGTCTCACCCCTCAACCAC290.21638561408744966No Hit
ATTGCGCAATGTGTCGAGGGGGAAGGAAGGTGGCCTTCGCTCTATATTCA290.21638561408744966No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC290.21638561408744966No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGCGCTTAGACTCG270.20146246828831518No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT270.20146246828831518No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACCGCGCTTAGAATCT260.19400089538874793No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCGCGCTTAGAATCGCG260.19400089538874793No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG260.19400089538874793No Hit
ATTGCGCAATGGATCCATGGGGATGAAGGGTGCATCAGATGGCGGTATCT260.19400089538874793No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACCGCGCTTA260.19400089538874793No Hit
ATTGCGCAATGGTACTTGTGGGGTGGTAATTGGGTCGTTTGCTAGCGTGC250.1865393224891807No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTGT240.17907774958961348No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC240.17907774958961348No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCGC230.17161617669004625No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC230.17161617669004625No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCGCGCTTAGAATCTCG220.16415460379047903No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGCG220.16415460379047903No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC210.1566930308909118No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGT200.14923145799134457No Hit
ATTGCGCAATGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGCGC190.14176988509177735No Hit
ATTGCGCAATGGCCGCAGAGGGACGGAATGGCCGCCCGATCATCATTTGT190.14176988509177735No Hit
ATTGCGCAATGTCGAGCGTGGGATACCGAAGCAGAATTCGGTAAGCGTTG190.14176988509177735No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTCT180.13430831219221012No Hit
ATTGCGCAATGCTGACGCGGGGGAGGCCGAGTACATCAAGCTCATTGAGG180.13430831219221012No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCT170.1268467392926429No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGGC170.1268467392926429No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGCGCTTAGAAGCG160.11938516639307566No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTG160.11938516639307566No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT160.11938516639307566No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGTC160.11938516639307566No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG160.11938516639307566No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGCT150.11192359349350843No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGT150.11192359349350843No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGCGCTTAGAATGG140.1044620205939412No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)