FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007822682

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822682
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13402
Sequences flagged as poor quality0
Sequence length151
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAAAGTACGAGAGTGTA12269.147888374869423No Hit
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT4113.066706461722131No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC2772.0668556931801225No Hit
CTTATACACATCTGACGCTGCCGACGAAAGTACGAGAGTGTAGATCTCGG1751.3057752574242651No Hit
CATCTGACGCTGCCGACGAAAGTACGAGAGTGTAGATCTCGGTGGTCGCC1481.1043127891359499Illumina Single End PCR Primer 1 (96% over 25bp)
CTCTTATACACATCTGACGCTGCCGACGAAAGTACGAGAGTGTAGATCTC1100.8207730189523952No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAAAGTACGAGA960.7163109983584539No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGAAAGTACGAGAGTG760.5670795403671094No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT740.5521563945679749No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAAAGTA730.5446948216684077No Hit
ATACACATCTGACGCTGCCGACGAAAGTACGAGAGTGTAGATCTCGGTGG550.4103865094761976No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGAAAGTACGAGAGTGTAGA440.32830920758095805No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT440.32830920758095805No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA410.3059244888822564No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC390.2910013430831219No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG390.2910013430831219No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG370.27607819728398747No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA360.26861662438442024No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG350.261155051484853No Hit
CATCTGACGCTGCCGACGAAAGTACGAGAGTGTAGATCTCGGTGGGCGCC350.261155051484853No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC330.24623190568571854No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAA300.22384718698701686No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC300.22384718698701686No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG290.21638561408744966No Hit
GATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAAAGTAC270.20146246828831518No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGC270.20146246828831518No Hit
ACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAAAGTA260.19400089538874793No Hit
TGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAAAGTACGAG260.19400089538874793No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC260.19400089538874793No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAAAGT250.1865393224891807No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA230.17161617669004625No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCA220.16415460379047903No Hit
CATCTGACGCTGCCGACGAAAGTACGAGAGTGTAGATCTCGGTGGTCGGC210.1566930308909118Illumina Single End PCR Primer 1 (95% over 23bp)
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATAT180.13430831219221012No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAA180.13430831219221012No Hit
CATCTGACGCTGCCGACGAAAGTACGAGAGTGTAGATCTCGGGGGTCGCC180.13430831219221012No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180.13430831219221012No Hit
CTTATACACATCTGACGCTGCCGACGAAAGTACGAGAGTGTAGTTCTCGG170.1268467392926429No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC170.1268467392926429No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT170.1268467392926429No Hit
CATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAAAGTAC160.11938516639307566No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGA160.11938516639307566No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATATTT150.11192359349350843No Hit
ATCTATCTCTTATACACATCTGACGCTGCCGACGAAAGTACGAGAGTGTA140.1044620205939412No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)