FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007822689

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822689
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14615
Sequences flagged as poor quality0
Sequence length151
%GC58

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATAGGTTGGATCG5583.817995210400274No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATAGGTTGGATCT4403.010605542251112No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC4242.9011289770783444No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC2621.792678754704071No Hit
ATTGCGCAATGATCAGAGAGGGGAAGCAAATCAAGAATTATTAACATTAA1130.7731782415326719No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA990.6773862470065002No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATAGGTTG950.6500171057133082No Hit
ATTGCGCAATGCAACGTTCGGGGTAGGACAATGGTAGGAGAGCGTTCTAA850.5815942524803285No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTATA820.5610673965104345No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATAGGTTGGAT810.5542251111871366No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG770.5268559698939446No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC690.4721176873075607No Hit
ATTGCGCAATGATTATGCCGGGATGTTACAGAGGAGGAAAACTAAATGTT650.4447485460143688No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC520.35579883681149505No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT460.31474512487170714No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT440.3010605542251112No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT440.3010605542251112No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT390.2668491276086213No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA360.24632227163872733No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATAG340.23263770099213138No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC340.23263770099213138No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG310.21211084502223743No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG300.20526855969893945No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT280.19158398905234347No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAT270.1847417037290455No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG250.17105713308244952No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTATAGGTTGGATCG250.17105713308244952No Hit
ATTGCGCAATGGTTCTCCTGGGGATAGGATCAGCTCCTTGACCTCTGAGG250.17105713308244952No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATAGGTTGGATCG240.16421484775915154No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTATAGGTTGGATCGCG230.15737256243585357No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACTATAGGTT200.13684570646595962No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTGT200.13684570646595962No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATAGGGTGGATCG200.13684570646595962No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATA190.13000342114266164No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGTC190.13000342114266164No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATAGGTTGGAGCG190.13000342114266164No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG190.13000342114266164No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTG180.12316113581936367No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT180.12316113581936367No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTATAGGTTGGATCT180.12316113581936367No Hit
ATTGCGCAATGAGGCAGGCGGGGTGGGCTTCTCCCCTCTCTTCCTAAATG180.12316113581936367No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG170.11631885049606569No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATAGGGTGGATCT170.11631885049606569No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGGTG160.1094765651727677No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGCT160.1094765651727677No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTGT160.1094765651727677No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC160.1094765651727677No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGAGGTTGGATCG160.1094765651727677No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTATAGGTTGGATCTCG150.10263427984946973No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC150.10263427984946973No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATAGGTTGGATGG150.10263427984946973No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCT150.10263427984946973No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)