FastQCFastQC Report
Tue 7 May 2024
EGAF00007822690

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822690
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14615
Total Bases2.2 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAAAGTACGAGAGTGTA12508.552856654122477No Hit
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT5513.770099213137188No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC3252.223742730071844No Hit
CTTATACACATCTGACGCTGCCGACGAAAGTACGAGAGTGTAGATCTCGG2351.6079370509750255No Hit
CATCTGACGCTGCCGACGAAAGTACGAGAGTGTAGATCTCGGTGGTCGCC1621.1084502223742732Illumina Single End PCR Primer 1 (96% over 25bp)
CTCTTATACACATCTGACGCTGCCGACGAAAGTACGAGAGTGTAGATCTC1440.9852890865549093No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAAAGTACGAGA960.6568593910366062No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGAAAGTACGAGAGTG880.6021211084502224No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAAAGTA830.5679096818337325No Hit
ATACACATCTGACGCTGCCGACGAAAGTACGAGAGTGTAGATCTCGGTGG650.4447485460143688No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT540.369483407458091No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT480.3284296955183031No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG460.31474512487170714No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC450.3079028395484092No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGAAAGTACGAGAGTGTAGA440.3010605542251112No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG430.29421826890181324No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC430.29421826890181324No Hit
CATCTGACGCTGCCGACGAAAGTACGAGAGTGTAGATCTCGGTGGGCGCC420.2873759835785152No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA360.24632227163872733No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG340.23263770099213138No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG330.22579541566883338No Hit
CATCTGACGCTGCCGACGAAAGTACGAGAGTGTAGATCTCGGTGGTCGGC320.2189531303455354Illumina Single End PCR Primer 1 (95% over 23bp)
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAAAGT300.20526855969893945No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT290.19842627437564148No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC270.1847417037290455No Hit
CTTATACACATCTGACGCTGCCGACGAAAGTACGAGAGTGTAGTTCTCGG270.1847417037290455No Hit
GATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAAAGTAC270.1847417037290455No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA250.17105713308244952No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC250.17105713308244952No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCA250.17105713308244952No Hit
GTCTCTTATACACATCTGACGCTGCCGACGAAAGTACGAGAGTGTAGATC240.16421484775915154No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATAT230.15737256243585357No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA220.1505302771125556No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAAAT210.1436879917892576No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAA210.1436879917892576No Hit
TGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAAAGTACGAG200.13684570646595962No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGA190.13000342114266164No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAAAATTT190.13000342114266164No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAA190.13000342114266164No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC190.13000342114266164No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGA180.12316113581936367No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCA180.12316113581936367No Hit
CATCTGACGCTGCCGACGAAAGTACGAGAGTGTAGATCTCGGGGGTCGCC180.12316113581936367No Hit
CGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180.12316113581936367No Hit
ACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAAAGTA170.11631885049606569No Hit
CATCTGACGCTGCCGACGAAAGTACGAGAGTGTAGATCTCGGTGGTGGCC170.11631885049606569Illumina Single End PCR Primer 1 (95% over 21bp)
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGCAA160.1094765651727677No Hit
TTTTTTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA160.1094765651727677No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGC160.1094765651727677No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAA150.10263427984946973No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAAATTT150.10263427984946973No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150.10263427984946973No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)