FastQCFastQC Report
Mon 16 Jan 2023
EGAF00007822693

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822693
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3931
Sequences flagged as poor quality0
Sequence length151
%GC59

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACTTATGGATCG2185.5456626812515895No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACTTATGGATCT1413.5868735690663955No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC1283.2561689137624015No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC651.6535232765199697No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACTTATGGAT390.9921139659119816No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACTTATG280.7122869498855253No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG280.7122869498855253No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT210.5342152124141439No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAC180.4578987534978377No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA180.4578987534978377No Hit
ATTGCGCAATGCTCACCGTGGGGGGCGCACGGTGGATGCCTTGGCACTAG180.4578987534978377No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC170.43245993385906895No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA150.3815822945815314No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG150.3815822945815314No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACTTAT130.3307046553039939No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT120.30526583566522514No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT110.2798270160264564No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT110.2798270160264564No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC100.2543881963876876No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACTTATGGAGCG100.2543881963876876No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG90.22894937674891885No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATGGATCG90.22894937674891885No Hit
ATTGCGCAATGAGATACTCGGGGGTTCCAGGAAATAGCCCCTCCATTATA80.2035105571101501No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGTCT80.2035105571101501No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCA80.2035105571101501No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACT80.2035105571101501No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGTC80.2035105571101501No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT70.17807173747138133No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACTTATGGATCGCG70.17807173747138133No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACCCACTTATGGATCG70.17807173747138133No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC70.17807173747138133No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG60.15263291783261257No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTC60.15263291783261257No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTG60.15263291783261257No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACCCACTTATGGATCT60.15263291783261257No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGT60.15263291783261257No Hit
ATTGCGCAATGGCACTCCCGGGTCAAACTTGAGAGTTTGATCCTGGCTCA60.15263291783261257No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT50.1271940981938438No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGT50.1271940981938438No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC50.1271940981938438No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGGCTGTCT50.1271940981938438No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCGGCCTG50.1271940981938438No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGGTGG40.10175527855507505No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC40.10175527855507505No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGT40.10175527855507505No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACTTATGGATCTCG40.10175527855507505No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGGCT40.10175527855507505No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTGT40.10175527855507505No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGATG40.10175527855507505No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTGT40.10175527855507505No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCGGCCGG40.10175527855507505No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGC40.10175527855507505No Hit
GGGGGGTGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG40.10175527855507505No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTCT40.10175527855507505No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCAGGAGACCCACTTATGGATCG40.10175527855507505No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGC40.10175527855507505No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGTG40.10175527855507505No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGGTG40.10175527855507505No Hit
ATTGCGCAATGTATGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGA40.10175527855507505No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGGTGCT40.10175527855507505No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)