FastQCFastQC Report
Mon 16 Jan 2023
EGAF00007822694

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822694
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3931
Sequences flagged as poor quality0
Sequence length151
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAAAGTACGAGAGTGTA41910.658865428644111No Hit
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT1573.9938946832866953No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC842.1368608496565757No Hit
CTTATACACATCTGACGCTGCCGACGAAAGTACGAGAGTGTAGATCTCGG451.1447468837445942No Hit
CTCTTATACACATCTGACGCTGCCGACGAAAGTACGAGAGTGTAGATCTC421.0684304248282879No Hit
CATCTGACGCTGCCGACGAAAGTACGAGAGTGTAGATCTCGGTGGTCGCC411.0429916051895192Illumina Single End PCR Primer 1 (96% over 25bp)
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGAAAGTACGAGAGTG411.0429916051895192No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAAAGTACGAGA340.8649198677181379No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC220.5596540320529128No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAAAGTA210.5342152124141439No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210.5342152124141439No Hit
ATACACATCTGACGCTGCCGACGAAAGTACGAGAGTGTAGATCTCGGTGG200.5087763927753752No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC200.5087763927753752No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGAAAGTACGAGAGTGTAGA160.4070211142203002No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130.3307046553039939No Hit
TGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAAAGTACGAG130.3307046553039939No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG120.30526583566522514No Hit
CATCTGACGCTGCCGACGAAAGTACGAGAGTGTAGATCTCGGTGGGCGCC120.30526583566522514No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA120.30526583566522514No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG110.2798270160264564No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGCA100.2543881963876876No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG90.22894937674891885No Hit
CATCTGACGCTGCCGACGAAAGTACGAGAGTGTAGATCTCGGGGGTCGCC90.22894937674891885No Hit
CATCTGACGCTGCCGACGAAAGTACGAGAGTGTAGATCTCGGTGGTCGGC90.22894937674891885Illumina Single End PCR Primer 1 (95% over 23bp)
GATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAAAGTAC80.2035105571101501No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGC80.2035105571101501No Hit
CATCTGACGCTGCCGACGAAAGTACGAGAGTGTAGATCTCGGTGGTGGCC80.2035105571101501Illumina Single End PCR Primer 1 (95% over 21bp)
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA80.2035105571101501No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC80.2035105571101501No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAAAGT70.17807173747138133No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAAAA70.17807173747138133No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAAAA70.17807173747138133No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC60.15263291783261257No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAA60.15263291783261257No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGAA60.15263291783261257No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAAA60.15263291783261257No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGA60.15263291783261257No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCCAAA50.1271940981938438No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT50.1271940981938438No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA50.1271940981938438No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGCAAT50.1271940981938438No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAAAGTACGAGTGTGTA50.1271940981938438No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAATAT50.1271940981938438No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG50.1271940981938438No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGCAAAATTT50.1271940981938438No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATCTG50.1271940981938438No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATAT50.1271940981938438No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGAAAAAT50.1271940981938438No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAAA50.1271940981938438No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAAT50.1271940981938438No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAA40.10175527855507505No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGCAA40.10175527855507505No Hit
CATCTGACGCTGCCGACGAAAGTACGAGAGTGTAGTTCTCGGTGGTCGCC40.10175527855507505No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATATTTAT40.10175527855507505No Hit
GTCTCTTATACACATCTGACGCTGCCGACGAAAGTACGAGAGTGTAGATC40.10175527855507505No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGAAA40.10175527855507505No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGAAAAA40.10175527855507505No Hit
CTCTTATACACATCTGACGCTGCCGACGAAAGTACGAGAGTGTAGTTCTC40.10175527855507505No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAAATGTA40.10175527855507505No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAA40.10175527855507505No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGCAATA40.10175527855507505No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGAAAA40.10175527855507505No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAAAAT40.10175527855507505No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCC40.10175527855507505No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC40.10175527855507505No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGAAA40.10175527855507505No Hit
CATCTGACGCTGCCGACGAAAGTACGAGAGTGTAGATCTCGGTGGGCGGC40.10175527855507505No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGAA40.10175527855507505No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATA40.10175527855507505No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT40.10175527855507505No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCAAAATTTA40.10175527855507505No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAAATGT40.10175527855507505No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAAT40.10175527855507505No Hit
CTTATACACATCTGACGCTGCCGACGAAAGTACGAGAGTGTAGTTCTCGG40.10175527855507505No Hit
GCAGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGAC40.10175527855507505No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAAAATGTG40.10175527855507505No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)