FastQCFastQC Report
Tue 7 May 2024
EGAF00007822698

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822698
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11126
Total Bases1.6 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAAAGTACGAGAGTGTA9158.22397986697825No Hit
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT3473.118820780154593No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC2402.1571094733057703No Hit
CTTATACACATCTGACGCTGCCGACGAAAGTACGAGAGTGTAGATCTCGG1641.474024806758943No Hit
CTCTTATACACATCTGACGCTGCCGACGAAAGTACGAGAGTGTAGATCTC1141.0246269998202409No Hit
CATCTGACGCTGCCGACGAAAGTACGAGAGTGTAGATCTCGGTGGTCGCC1090.9796872191263706Illumina Single End PCR Primer 1 (96% over 25bp)
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAAAGTACGAGA820.7370124033794715No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAAAGTA610.5482653244652166No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGAAAGTACGAGAGTG560.5033255437713464No Hit
ATACACATCTGACGCTGCCGACGAAAGTACGAGAGTGTAGATCTCGGTGG520.4673737192162502No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT450.4044580262448319No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG440.3954700701060579No Hit
CATCTGACGCTGCCGACGAAAGTACGAGAGTGTAGATCTCGGTGGGCGCC360.3235664209958656No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC350.3145784648570915No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC330.29660255257954343No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA320.28761459644076937No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG290.2606507280244472No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGAAAGTACGAGAGTGTAGA280.2516627718856732No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC260.2336868596081251No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG260.2336868596081251No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAA250.22469890346935106No Hit
CATCTGACGCTGCCGACGAAAGTACGAGAGTGTAGATCTCGGTGGTCGGC230.20672299119180296Illumina Single End PCR Primer 1 (95% over 23bp)
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT230.20672299119180296No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA210.1887470789142549No Hit
TGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAAAGTACGAG200.17975912277548087No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG200.17975912277548087No Hit
CATCTGACGCTGCCGACGAAAGTACGAGAGTGTAGATCTCGGGGGTCGCC190.1707711666367068No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA180.1617832104979328No Hit
CTTATACACATCTGACGCTGCCGACGAAAGTACGAGAGTGTAGTTCTCGG180.1617832104979328No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC170.15279525435915872No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCA170.15279525435915872No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC170.15279525435915872No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGC160.14380729822038468No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATA150.13481934208161064No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAAAGT150.13481934208161064No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCA140.1258313859428366No Hit
CATCTGACGCTGCCGACGAAAGTACGAGAGTGTAGATCTCGGTGGTGGCC140.1258313859428366Illumina Single End PCR Primer 1 (95% over 21bp)
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCG140.1258313859428366No Hit
CATCTGACGCTGCCGACGAAAGTACGAGAGTGTAGTTCTCGGTGGTCGCC130.11684342980406255No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT130.11684342980406255No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAAA120.10785547366528853No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGA120.10785547366528853No Hit
GTCTCTTATACACATCTGACGCTGCCGACGAAAGTACGAGAGTGTAGATC120.10785547366528853No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAA120.10785547366528853No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC120.10785547366528853No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120.10785547366528853No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)