FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007822699

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822699
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13793
Sequences flagged as poor quality0
Sequence length151
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACATGGACGATCG5904.2775320814906115No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACATGGACGATCT3592.602769520771406No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC3232.3417675632567243No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC2241.6240121800913505No Hit
ATTGCGCAATGCTATCCGCGGGGACCGAACCGTTCAATGTTGAAAAATTG850.6162546219096643No Hit
ATTGCGCAATGGAAGTACCGGGGGTGAAGTTTCTAAAAGAAGAGTGAGAT790.5727542956572175No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACATGGACGAT770.5582541869064017No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACATGGAC720.5220039150293627No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC710.5147538606539549No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTACA670.4857536431523236No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA670.4857536431523236No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG550.39875299064742986No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT540.39150293627202204No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT540.39150293627202204No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTACATGGACGATCG510.36975277314579863No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT380.275502066265497No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC350.25375190313927354No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC340.24650184876386572No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACATGGACGAGCG330.23925179438845792No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT320.2320017400130501No Hit
ATTGCGCAATGCGTACATGGGGGGTAGCGGTGACGACTCGTGTATGAAGG320.2320017400130501No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA310.22475168563764225No Hit
ATTGCGCAATGTAAGGTATGGGGAGCTATACTCTCAAATCACACTTCTCC310.22475168563764225No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG290.21025157688682664No Hit
ATTGCGCAATGCAGGAACCGGGGCCACGCAAGCCTCTCACCCTTAGCTTT280.2030015225114188No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACAT270.19575146813601102No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC260.1885014137606032No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG260.1885014137606032No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTACATGGACGATCT240.17400130500978758No Hit
ATTGCGCAATGAGGGCGCTGGGCAAAAGAAACAATTGAACAAGAGAAGCA220.15950119625897194No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC220.15950119625897194No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG220.15950119625897194No Hit
ATTGCGCAATGAGCGGTTGGGGGGAATTTCGCGTGAATCTGGCGGGACCA200.1450010875081563No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTACATGGACGATCTCG190.1377510331327485No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT190.1377510331327485No Hit
ATTGCGCAATGTTAAACGTGGGAGGGATTGCCTTAGTAACGGCGAGTGAA190.1377510331327485No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAC180.13050097875734068No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACA180.13050097875734068No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTG180.13050097875734068No Hit
ATTGCGCAATGTGCGTAACGGGACAATCGCACCTGGCAGCATCGTCACGC180.13050097875734068No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACTACATGGA180.13050097875734068No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC170.12325092438193286No Hit
ATTGCGCAATGGCGTCCTGGGGGAGCCTGGGGGAACAGGAGGGATTATAC170.12325092438193286No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTC170.12325092438193286No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTCT170.12325092438193286No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG170.12325092438193286No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT170.12325092438193286No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTGT170.12325092438193286No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTG160.11600087000652505No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG160.11600087000652505No Hit
ATTGCGCAATGGTAGTCACGGGATCGACATGTGAATTCATAGCATGTCAG150.10875081563111724No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGT140.1015007612557094No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCTT140.1015007612557094No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGTC140.1015007612557094No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTACATGGACGATCGCG140.1015007612557094No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)