FastQCFastQC Report
Tue 7 May 2024
EGAF00007822700

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822700
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13793
Total Bases2 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAAAGTACGAGAGTGTA10947.931559486696151No Hit
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT4403.190023925179439No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC2751.9937649532371493No Hit
CTTATACACATCTGACGCTGCCGACGAAAGTACGAGAGTGTAGATCTCGG1991.4427608207061553No Hit
CATCTGACGCTGCCGACGAAAGTACGAGAGTGTAGATCTCGGTGGTCGCC1461.058507938809541Illumina Single End PCR Primer 1 (96% over 25bp)
CTCTTATACACATCTGACGCTGCCGACGAAAGTACGAGAGTGTAGATCTC1060.7685057637932284No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAAAGTACGAGA790.5727542956572175No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGAAAGTACGAGAGTG740.5365040237801784No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAAAGTA650.47125353440150797No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT540.39150293627202204No Hit
ATACACATCTGACGCTGCCGACGAAAGTACGAGAGTGTAGATCTCGGTGG480.34800261001957516No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT440.3190023925179439No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG400.2900021750163126No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG400.2900021750163126No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC350.25375190313927354No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA330.23925179438845792No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGAAAGTACGAGAGTGTAGA330.23925179438845792No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAA330.23925179438845792No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC320.2320017400130501No Hit
CATCTGACGCTGCCGACGAAAGTACGAGAGTGTAGATCTCGGTGGTCGGC320.2320017400130501Illumina Single End PCR Primer 1 (95% over 23bp)
CATCTGACGCTGCCGACGAAAGTACGAGAGTGTAGATCTCGGTGGGCGCC320.2320017400130501No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG280.2030015225114188No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC270.19575146813601102No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT240.17400130500978758No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC240.17400130500978758No Hit
ACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAAAGTA230.16675125063437976No Hit
CTTATACACATCTGACGCTGCCGACGAAAGTACGAGAGTGTAGTTCTCGG230.16675125063437976No Hit
GATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAAAGTAC200.1450010875081563No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCA200.1450010875081563No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG190.1377510331327485No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA180.13050097875734068No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGCA180.13050097875734068No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA180.13050097875734068No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC180.13050097875734068No Hit
CATCTGACGCTGCCGACGAAAGTACGAGAGTGTAGATCTCGGGGGTCGCC180.13050097875734068No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAAAGT170.12325092438193286No Hit
TGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAAAGTACGAG160.11600087000652505No Hit
GTCTCTTATACACATCTGACGCTGCCGACGAAAGTACGAGAGTGTAGATC150.10875081563111724No Hit
CTCTTATACACATCTGACGCTGCCGACGAAAGTACGAGAGTGTAGTTCTC150.10875081563111724No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGC150.10875081563111724No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAAAT140.1015007612557094No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA140.1015007612557094No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)