FastQCFastQC Report
Tue 7 May 2024
EGAF00007822703

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822703
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12623
Total Bases1.9 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC59

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCACGGATCG5714.523488869523884No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC4723.7392062108848925No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCACGGATCT3222.550899152340965No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC2862.265705458290422No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG720.5703873881010854No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCACG710.5624653410441258No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCACGGAT660.5228551057593283No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC660.5228551057593283No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA650.5149330587023687No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCA640.5070110116454092No Hit
ATTGCGCAATGATGGAAACGGGGAGCCTGGGGGAACAGGTAGGTCACTTT600.4753228234175711No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT450.35649211756317833No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT390.30895983522142123No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTTCACCACGGATCG390.30895983522142123No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT360.2851936940505427No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC350.27727164699358314No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA260.20597322348094746No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT240.19012912936702844No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC240.19012912936702844No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG240.19012912936702844No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG220.1742850352531094No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCAC220.1742850352531094No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC200.15844094113919036No Hit
ATTGCGCAATGGCTCACATGGGGCTTTTTTTCCTCAGCTGCCGCCAAGGT200.15844094113919036No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCACGGAGCG200.15844094113919036No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC200.15844094113919036No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG190.15051889408223085No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCACGGAGCT170.1346747999683118No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG170.1346747999683118No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGCT160.1267527529113523No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT160.1267527529113523No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTGT160.1267527529113523No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCACGGATCGCG160.1267527529113523No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG160.1267527529113523No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCT160.1267527529113523No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTCT150.11883070585439277No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCT150.11883070585439277No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT150.11883070585439277No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC150.11883070585439277No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGT140.11090865879743327No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGGCT140.11090865879743327No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTG140.11090865879743327No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTC130.10298661174047373No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)