FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007822717

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822717
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15823
Sequences flagged as poor quality0
Sequence length151
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCATCACTATATCG7124.4997788030082795No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCATCACTATATCT4352.7491626113884853No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC4022.5406054477659104No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC2311.4599001453580231No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCATCACTATAT940.5940719206218795No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACCCATCACTATATCG930.5877520065727105No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA810.5119130379826834No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCATCACTA800.5055931239335145No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAT760.48031346773683875No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG690.4360740693926563No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC670.42343424129431845No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT570.36023510080262905No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT430.27175630411426405No Hit
ATTGCGCAATGGTGGGTTCGGGCAGGAGCTGGCTAAGTGCAAGATCGACA420.2654363900650951No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACCCATCACTATATCT380.24015673386841938No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT340.2148770776717437No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC300.18959742147506795No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAT280.17695759337673006No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCA280.17695759337673006No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA280.17695759337673006No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG280.17695759337673006No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG270.17063767932756116No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCATCACTATATGG260.16431776527839223No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT250.15799785122922327No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG250.15799785122922327No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG240.15167793718005435No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCATCACT240.15167793718005435No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT230.14535802313088542No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCCATCACTATATCGCG220.1390381090817165No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC220.1390381090817165No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC210.13271819503254756No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCATCACTATAGCG200.12639828098337863No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCATCACTATCTCG200.12639828098337863No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCATCACTCTATCG180.11375845288504076No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGT170.10743853883587184No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGT170.10743853883587184No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCATCACGATATCG170.10743853883587184No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCATCACTAGATCG160.1011186247867029No Hit
ATTGCGCAATGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCATC160.1011186247867029No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCATC160.1011186247867029No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG160.1011186247867029No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)