FastQCFastQC Report
Tue 7 May 2024
EGAF00007822718

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822718
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15823
Total Bases2.3 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC53

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATGGACACTCCGTGTA15539.81482651835935No Hit
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT5203.2863553055678443No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC3061.933893699045693No Hit
CTTATACACATCTGACGCTGCCGACGATGGACACTCCGTGTAGATCTCGG2781.7569361056689627No Hit
CTCTTATACACATCTGACGCTGCCGACGATGGACACTCCGTGTAGATCTC1651.0427858181128737No Hit
CATCTGACGCTGCCGACGATGGACACTCCGTGTAGATCTCGGTGGTCGCC1550.9795866776211843Illumina RNA PCR Primer (100% over 22bp)
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGGACACTCCGTG1040.6572710611135689No Hit
ATACACATCTGACGCTGCCGACGATGGACACTCCGTGTAGATCTCGGTGG860.5435126082285281No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGGACACTCC850.5371926941793592No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGGAC830.5245528660810214No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT590.37287492890096696No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGATGGACACTCCGTGTAGA530.3349554446059534No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT470.29703596031093976No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC410.2591164760159262No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG390.24647664791758833No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC390.24647664791758833No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG390.24647664791758833No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG380.24015673386841938No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC320.2022372495734058No Hit
CATCTGACGCTGCCGACGATGGACACTCCGTGTAGATCTCGGTGGGCGCC310.19591733552423687Illumina RNA PCR Primer (95% over 22bp)
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC310.19591733552423687No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA290.18327750742589902No Hit
ACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGGAC290.18327750742589902No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAT290.18327750742589902No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA280.17695759337673006No Hit
TGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGGACACTC280.17695759337673006No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGGA280.17695759337673006No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG240.15167793718005435No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT230.14535802313088542No Hit
CATCTGACGCTGCCGACGATGGACACTCCGTGTAGATCTCGGTGGTCGGC220.1390381090817165Illumina RNA PCR Primer (95% over 22bp)
GTCTCTTATACACATCTGACGCTGCCGACGATGGACACTCCGTGTAGATC210.13271819503254756No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAA200.12639828098337863No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGA200.12639828098337863No Hit
CGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200.12639828098337863No Hit
TCTGTCTCTTATACACATCTGACGCTGCCGACGATGGACACTCCGTGTAG190.12007836693420969No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA180.11375845288504076No Hit
CATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGGACA180.11375845288504076No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCA180.11375845288504076No Hit
CATCTGACGCTGCCGACGATGGACACTCCGTGTAGATCTCGGGGGTCGCC180.11375845288504076Illumina RNA PCR Primer (95% over 22bp)
CTTATACACATCTGACGCTGCCGACGATGGACACTCCGTGTAGTTCTCGG180.11375845288504076No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAA170.10743853883587184No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC160.1011186247867029No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)