FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007822721

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822721
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18573
Sequences flagged as poor quality0
Sequence length151
%GC57

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACATCGCATATCG9064.878048780487805No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACATCGCATATCT6523.5104721908146237No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC4632.4928659882625315No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC3521.8952242502557475No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG1260.6784041350347279No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1260.6784041350347279No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACATCGCATAT1200.6460991762235503No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC1150.6191783772142357No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACATCGCA1050.5653367791956065No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTACA920.49534270177138856No Hit
ATTGCGCAATGCAGCGCCGGGGAAGAGCACGTGGTAGGAGAGCGTTCTAA820.44150110375275936No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT770.41458030474344476No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT720.3876595057341302No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTACATCGCATATCG720.3876595057341302No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT700.37689118613040434No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA500.26920799009314594No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC480.2584396704894201No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG460.24767135088569428No Hit
AGCAGAGCCTGGGCAACAAGAGTGAACTCCGTCTCAAAAAAAAAAAAAAA380.20459807247079093No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT380.20459807247079093No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC360.1938297528670651No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACATCGCATAGCG360.1938297528670651No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG350.18844559306520217No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC350.18844559306520217No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACA330.17767727346147633No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG320.17229311365961342No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTACATCGCATATCGCG310.1669089538577505No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTC310.1669089538577505No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAC310.1669089538577505No Hit
ATTGCGCAATGGCATGTACGGGGGGTGGGGTTTGGAAGCTTCTGGAAGTC310.1669089538577505No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC310.1669089538577505No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTACATCGCATATCT310.1669089538577505No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG310.1669089538577505No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGT300.16152479405588757No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACAT280.15075647445216173No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTG280.15075647445216173No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC260.1399881548484359No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTG260.1399881548484359No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACTACATCGC260.1399881548484359No Hit
ATTGCGCAATGGAGCTCACGGGCGCGGTAATACGAAGGGGGCTAGCGTTG240.12921983524471006No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT230.12383567544284714No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT230.12383567544284714No Hit
ATTGCGCAATGGCATGTATGGGGGGTGGGGTTTGGAAGCTTCTGGAAGTC230.12383567544284714No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACATCGCATATGG210.11306735583912131No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTCT210.11306735583912131No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGCT200.10768319603725839No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACATCGCAGATCG200.10768319603725839No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGTC190.10229903623539546No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)