FastQCFastQC Report
Tue 7 May 2024
EGAF00007822722

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822722
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18573
Total Bases2.8 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC52

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATGGACACTCCGTGTA17519.427663813061972No Hit
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT6273.3758681957680503No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC4332.331341194206644No Hit
CTTATACACATCTGACGCTGCCGACGATGGACACTCCGTGTAGATCTCGG2941.5829429817476985No Hit
CATCTGACGCTGCCGACGATGGACACTCCGTGTAGATCTCGGTGGTCGCC1820.9799170839390513Illumina RNA PCR Primer (100% over 22bp)
CTCTTATACACATCTGACGCTGCCGACGATGGACACTCCGTGTAGATCTC1670.8991546869111076No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGGACACTCCGTG1340.7214774134496312No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGGACACTCC1190.6407150164216874No Hit
ATACACATCTGACGCTGCCGACGATGGACACTCCGTGTAGATCTCGGTGG930.5007268615732515No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGGAC860.46303774296021105No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT760.40919614494158185No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG750.40381198513971894No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT730.3930436655359931No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA600.32304958811177514No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC550.29612878910246054No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGATGGACACTCCGTGTAGA530.2853604694987347No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG520.2799763096968718No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC510.2745921498950089No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC460.24767135088569428No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG440.23690303128196843No Hit
CATCTGACGCTGCCGACGATGGACACTCCGTGTAGATCTCGGTGGGCGCC420.22613471167824262Illumina RNA PCR Primer (95% over 22bp)
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC410.22075055187637968No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT400.21536639207451677No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC360.1938297528670651No Hit
CATCTGACGCTGCCGACGATGGACACTCCGTGTAGATCTCGGTGGTGGCC360.1938297528670651Illumina RNA PCR Primer (95% over 22bp)
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAT360.1938297528670651No Hit
ACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGGAC350.18844559306520217No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG330.17767727346147633No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA300.16152479405588757No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCA290.15614063425402466No Hit
TGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGGACACTC290.15614063425402466No Hit
CATCTGACGCTGCCGACGATGGACACTCCGTGTAGATCTCGGGGGTCGCC290.15614063425402466Illumina RNA PCR Primer (95% over 22bp)
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAA280.15075647445216173No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA270.14537231465029882No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGGA260.1399881548484359No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGA250.13460399504657297No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCG240.12921983524471006No Hit
GTCTCTTATACACATCTGACGCTGCCGACGATGGACACTCCGTGTAGATC230.12383567544284714No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGC230.12383567544284714No Hit
GATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGGACA230.12383567544284714No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCA220.11845151564098422No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAA210.11306735583912131No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGAAA210.11306735583912131No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATA210.11306735583912131No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)