FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007822723

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822723
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12957
Sequences flagged as poor quality0
Sequence length151
%GC58

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATAGGTTGGATCG5023.8743536312417994No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATAGGTTGGATCT3812.9404954850659877No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC3252.508296673612719No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC1991.5358493478428648No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG850.6560160530987111No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATAGGTTGGAT790.6097090375858609No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTATA730.5634020220730107No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATAGGTTG720.5556841861542023No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA690.5325306783977772No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC680.5248128424789689No Hit
ATTGCGCAATGCCGAGCCAGGGGACGCTAGCGACGACCATGCATAGCAAC570.43991664737207686No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT500.38589179594041834No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT450.3473026163463765No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT350.2701242571582928No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA330.25468858532067606No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTATAGGTTGGATCG330.25468858532067606No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC320.24697074940186772No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC300.23153507756425099No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAT240.18522806205140077No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTATAGGTTGGATCGCG230.17751022613259243No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG230.17751022613259243No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG230.17751022613259243No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG230.17751022613259243No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATAGGTTGGATCG220.16979239021378406No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG220.16979239021378406No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC220.16979239021378406No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATAG200.15435671837616732No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT200.15435671837616732No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT200.15435671837616732No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGTC190.14663888245735895No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTG170.13120321061974222No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACTATAGGTT170.13120321061974222No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATAGGTTGGAGCG170.13120321061974222No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATAGGGTGGATCT170.13120321061974222No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGT160.12348537470093386No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC160.12348537470093386No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC150.11576753878212549No Hit
GGGGTGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG150.11576753878212549No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT150.11576753878212549No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCGGT150.11576753878212549No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG150.11576753878212549No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATAGGGTGGATCG150.11576753878212549No Hit
ATTGCGCAATGGTCCAGAGGGGAATTTTGAACTAGCAAATCTGTTTCTTT140.10804970286331712No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGCTGTCTCTTA140.10804970286331712No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTATAGGTTGGATCTCG130.10033186694450877No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCT130.10033186694450877No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGCT130.10033186694450877No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTG130.10033186694450877No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCT130.10033186694450877No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)