FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007822725

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822725
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13425
Sequences flagged as poor quality0
Sequence length151
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCGCCATACTATCG5564.1415270018621975No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCGCCATACTATCT4573.404096834264432No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC3722.7709497206703912No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC2541.8919925512104283No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG1030.7672253258845437No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCGCCATACTAT940.7001862197392923No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCGC820.6108007448789572No Hit
ATTGCGCAATGAACGGAAGGGGGAAACGATGTGGGAAGGCATAGACAGCT790.5884543761638734No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCGCCATAC790.5884543761638734No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT700.521415270018622No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC660.49162011173184356No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA650.48417132216014896No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT580.43202979515828677No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACCCGCCATACTATCG510.3798882681564246No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT480.3575418994413408No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT380.28305400372439476No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCGCC360.2681564245810056No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG360.2681564245810056No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC320.23836126629422721No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACCCGCCATACTATCT300.22346368715083798No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC300.22346368715083798No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA300.22346368715083798No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG300.22346368715083798No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG290.21601489757914338No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGT280.2085661080074488No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC280.2085661080074488No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGT270.2011173184357542No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC260.19366852886405958No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG240.1787709497206704No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTG240.1787709497206704No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCCGCCATACTATCGCG230.1713221601489758No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTGT230.1713221601489758No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT220.1638733705772812No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG220.1638733705772812No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCGC210.1564245810055866No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTC200.14897579143389197No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCG200.14897579143389197No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCT200.14897579143389197No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT190.14152700186219738No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCGCCATA180.1340782122905028No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGCT170.1266294227188082No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGTC170.1266294227188082No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCC150.11173184357541899No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTG150.11173184357541899No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCCT150.11173184357541899No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGC140.1042830540037244No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC140.1042830540037244No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)