FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007822726

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822726
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13425
Sequences flagged as poor quality0
Sequence length151
%GC52

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATGGACACTCCGTGTA11238.364990689013036No Hit
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT4703.5009310986964617No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC3282.4432029795158288No Hit
CTTATACACATCTGACGCTGCCGACGATGGACACTCCGTGTAGATCTCGG2021.504655493482309No Hit
CATCTGACGCTGCCGACGATGGACACTCCGTGTAGATCTCGGTGGTCGCC1381.0279329608938548Illumina Single End PCR Primer 1 (95% over 22bp)
CTCTTATACACATCTGACGCTGCCGACGATGGACACTCCGTGTAGATCTC1020.7597765363128492No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGGACACTCCGTG940.7001862197392923No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGGACACTCC900.6703910614525139No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGGAC770.5735567970204841No Hit
ATACACATCTGACGCTGCCGACGATGGACACTCCGTGTAGATCTCGGTGG570.42458100558659223No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG540.4022346368715084No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT520.38733705772811916No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC410.3054003724394786No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG400.29795158286778395No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC360.2681564245810056No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG350.260707635009311No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG350.260707635009311No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGATGGACACTCCGTGTAGA340.2532588454376164No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA320.23836126629422721No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT320.23836126629422721No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC310.2309124767225326No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC300.22346368715083798No Hit
CATCTGACGCTGCCGACGATGGACACTCCGTGTAGATCTCGGTGGTCGGC280.2085661080074488Illumina RNA PCR Primer (95% over 22bp)
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC280.2085661080074488No Hit
CATCTGACGCTGCCGACGATGGACACTCCGTGTAGATCTCGGGGGTCGCC280.2085661080074488Illumina RNA PCR Primer (95% over 22bp)
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA230.1713221601489758No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAA230.1713221601489758No Hit
ACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGGAC220.1638733705772812No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT220.1638733705772812No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA200.14897579143389197No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCA200.14897579143389197No Hit
GATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGGACA200.14897579143389197No Hit
CATCTGACGCTGCCGACGATGGACACTCCGTGTAGATCTCGGTGGTGGCC190.14152700186219738Illumina RNA PCR Primer (95% over 22bp)
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAA190.14152700186219738No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGGA190.14152700186219738No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCA180.1340782122905028No Hit
ATCTATCTCTTATACACATCTGACGCTGCCGACGATGGACACTCCGTGTA170.1266294227188082No Hit
CATCTGACGCTGCCGACGATGGACACTCCGTGTAGATCTCGGTGGGCGCC160.11918063314711361Illumina RNA PCR Primer (95% over 22bp)
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGA160.11918063314711361No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGC160.11918063314711361No Hit
CTTATACACATCTGACGCTGCCGACGATGGACACTCCGTGTAGTTCTCGG160.11918063314711361No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATA150.11173184357541899No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAAA150.11173184357541899No Hit
TGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGGACACTC150.11173184357541899No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAT150.11173184357541899No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC140.1042830540037244No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)